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• W2012637627 abstract "The use of m-nitrobenzyl alcohol (m-NBA) to enhance charging of noncovalent complexes formed by electrospray ionization from aqueous solutions was investigated. Addition of up to 1% m-NBA can result in a significant increase in the average charging of complexes, ranging from ∼13% for the homo-heptamer of NtrC4-RC (317 kDa; maximum charge state increases from 42+ to 44+) to ∼49% for myoglobin (17.6 kDa; maximum charge state increases from 9+ to 16+). Charge state distributions of larger complexes obtained from heated solutions to which no m-NBA was added are remarkably similar to those containing small amounts of m-NBA. Dissociation of the complexes through identical channels both upon addition of higher concentrations of m-NBA and heating is observed. These results indicate that the enhanced charging upon addition of m-NBA to aqueous electrospray solutions is a result of droplet heating owing to the high boiling point of m-NBA, which results in a change in the higher-order structure and/or dissociation of the complexes. For monomeric proteins and small complexes, the enhancement of charging is lower for heated aqueous solutions than from solutions with m-NBA because rapid folding of proteins from heated solutions that do not contain m-NBA can occur after the electrospray droplet is formed and is evaporatively cooled." @default.
• W2012637627 created "2016-06-24" @default.
• W2012637627 creator A5014296444 @default.
• W2012637627 creator A5055609661 @default.
• W2012637627 date "2009-07-03" @default.
• W2012637627 modified "2023-10-16" @default.
• W2012637627 title "Origin of supercharging in electrospray ionization of noncovalent complexes from aqueous solution" @default.
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• W2012637627 doi "https://doi.org/10.1016/j.jasms.2009.06.012" @default.
• W2012637627 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2789484" @default.
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