Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012662704> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2012662704 endingPage "17" @default.
- W2012662704 startingPage "9" @default.
- W2012662704 abstract "Violet laser diodes have become common and reliable laser sources for benchtop flow cytometers. While these lasers are very useful for a variety of violet and some ultraviolet-excited fluorochromes (e.g., DAPI), they do not efficiently excite most UV-stimulated probes. In this study, the next generation of InGaN near-UV laser diodes (NUVLDs) emitting in the 370-375-nm range have been evaluated as laser sources for cuvette-based flow cytometers.Several NUVLDs, ranging in wavelength from 370 to 374 nm and in power level from 1.5 to 10 mW, were mounted on a BD Biosciences LSR II and evaluated for their ability to excite cells labeled with the UV DNA binding dye DAPI, several UV phenotyping fluorochromes (including Alexa Fluor 350, Marina Blue, and quantum dots), and the fluorescent calcium chelator indo-1.NUVLDs at the 8-10-mW power range gave detection sensitivity levels comparable to more powerful solid-state and ion laser sources, using low-fluorescence microsphere beads as measurement standards. NUVLDs at all tested power levels allowed extremely high-resolution DAPI cell cycle analysis, and sources in the 8-10-mW power range excited Alexa Fluor 350, Marina Blue, and a variety of quantum dots at virtually the same signal-to-noise ratios as more powerful UV sources.These evaluations indicate that near-UV laser diodes installed on a cuvette-based flow cytometer performed nearly as well as more powerful solid-state UV lasers on the same instrumentation, and comparably to more powerful ion lasers on a jet-in-air system, and. Despite their limited power, integration of these small and inexpensive lasers into benchtop flow cytometers should allow the use of flow cytometric applications requiring UV excitation on a wide variety of instruments." @default.
- W2012662704 created "2016-06-24" @default.
- W2012662704 creator A5061239953 @default.
- W2012662704 date "2004-07-28" @default.
- W2012662704 modified "2023-10-10" @default.
- W2012662704 title "Analysis of UV-excited fluorochromes by flow cytometry using near-ultraviolet laser diodes" @default.
- W2012662704 cites W2029458955 @default.
- W2012662704 cites W2056639099 @default.
- W2012662704 cites W2062485439 @default.
- W2012662704 cites W2155648789 @default.
- W2012662704 doi "https://doi.org/10.1002/cyto.a.20032" @default.
- W2012662704 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15351984" @default.
- W2012662704 hasPublicationYear "2004" @default.
- W2012662704 type Work @default.
- W2012662704 sameAs 2012662704 @default.
- W2012662704 citedByCount "35" @default.
- W2012662704 countsByYear W20126627042012 @default.
- W2012662704 countsByYear W20126627042014 @default.
- W2012662704 countsByYear W20126627042015 @default.
- W2012662704 countsByYear W20126627042017 @default.
- W2012662704 countsByYear W20126627042018 @default.
- W2012662704 countsByYear W20126627042019 @default.
- W2012662704 countsByYear W20126627042020 @default.
- W2012662704 countsByYear W20126627042022 @default.
- W2012662704 crossrefType "journal-article" @default.
- W2012662704 hasAuthorship W2012662704A5061239953 @default.
- W2012662704 hasConcept C120665830 @default.
- W2012662704 hasConcept C121332964 @default.
- W2012662704 hasConcept C192562407 @default.
- W2012662704 hasConcept C2776759294 @default.
- W2012662704 hasConcept C2776798109 @default.
- W2012662704 hasConcept C37160838 @default.
- W2012662704 hasConcept C49040817 @default.
- W2012662704 hasConcept C520434653 @default.
- W2012662704 hasConcept C60760409 @default.
- W2012662704 hasConcept C78434282 @default.
- W2012662704 hasConcept C88815150 @default.
- W2012662704 hasConcept C91881484 @default.
- W2012662704 hasConceptScore W2012662704C120665830 @default.
- W2012662704 hasConceptScore W2012662704C121332964 @default.
- W2012662704 hasConceptScore W2012662704C192562407 @default.
- W2012662704 hasConceptScore W2012662704C2776759294 @default.
- W2012662704 hasConceptScore W2012662704C2776798109 @default.
- W2012662704 hasConceptScore W2012662704C37160838 @default.
- W2012662704 hasConceptScore W2012662704C49040817 @default.
- W2012662704 hasConceptScore W2012662704C520434653 @default.
- W2012662704 hasConceptScore W2012662704C60760409 @default.
- W2012662704 hasConceptScore W2012662704C78434282 @default.
- W2012662704 hasConceptScore W2012662704C88815150 @default.
- W2012662704 hasConceptScore W2012662704C91881484 @default.
- W2012662704 hasIssue "1" @default.
- W2012662704 hasLocation W20126627041 @default.
- W2012662704 hasLocation W20126627042 @default.
- W2012662704 hasOpenAccess W2012662704 @default.
- W2012662704 hasPrimaryLocation W20126627041 @default.
- W2012662704 hasRelatedWork W106927775 @default.
- W2012662704 hasRelatedWork W1973358872 @default.
- W2012662704 hasRelatedWork W2012662704 @default.
- W2012662704 hasRelatedWork W2019216657 @default.
- W2012662704 hasRelatedWork W2028252080 @default.
- W2012662704 hasRelatedWork W2083911186 @default.
- W2012662704 hasRelatedWork W2125703222 @default.
- W2012662704 hasRelatedWork W2154311086 @default.
- W2012662704 hasRelatedWork W2426486745 @default.
- W2012662704 hasRelatedWork W4242672724 @default.
- W2012662704 hasVolume "61A" @default.
- W2012662704 isParatext "false" @default.
- W2012662704 isRetracted "false" @default.
- W2012662704 magId "2012662704" @default.
- W2012662704 workType "article" @default.