FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012711488> ?p ?o ?g. }

• W2012711488 endingPage "1068" @default.
• W2012711488 startingPage "1058" @default.
• W2012711488 abstract "Abstract Polyelectrolyte complexes have been widely studied as gene carriers in recent years. In this study, poly (ethylene imine) was grafted onto chitosan (PEI‐ g ‐CHI) as a nonviral gene carrier in order to improve the water solubility as well as the inherent transfection efficiency of chitosan. We present a novel method to conjugate the amine or hydroxyl groups of chitosan (CHI) and the amine groups of PEI through opening the epoxide rings of ethylene glycol diglycidyl ether (EX‐810), which also brings the merits as mentioned in PEGylation chemistry. The degree of substitution of PEI onto CHI was characterized by NMR. The preliminarily cellular mechanisms, from the cellular entry to the endosomal release, were investigated by the correlations among the physicochemical properties of the DNA‐polymer complexes, the buffering capacity of the modified polymer, the cytotoxicity, and the efficiency of the transgene expression. The cytotoxicity assayed by MTT shows that cell viability of PEI‐ g ‐CHI is higher than CHI especially noticeable at high concentrations using human kidney 293T cells. The efficiency of transgene expression and the amount of intracellular plasmid were monitored using green fluorescent protein (GFP) and visualized by fluorescence microscopy. The transfection efficiency of PEI‐ g ‐CHI/DNA polyplex is significantly better than CHI/DNA polyplex when using the weight ratios higher than 2.5. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009" @default.
• W2012711488 created "2016-06-24" @default.
• W2012711488 creator A5011916328 @default.
• W2012711488 creator A5017864062 @default.
• W2012711488 creator A5047609980 @default.
• W2012711488 creator A5050822671 @default.
• W2012711488 creator A5081601133 @default.
• W2012711488 date "2008-04-10" @default.
• W2012711488 modified "2023-10-17" @default.
• W2012711488 title "Poly(ethylene imine)-<i>g</i>-chitosan using EX-810 as a spacer for nonviral gene delivery vectors" @default.
• W2012711488 cites W1580847663 @default.
• W2012711488 cites W1969532639 @default.
• W2012711488 cites W1973459809 @default.
• W2012711488 cites W1975600099 @default.
• W2012711488 cites W1975819598 @default.
• W2012711488 cites W1977531943 @default.
• W2012711488 cites W1982968590 @default.
• W2012711488 cites W1985610066 @default.
• W2012711488 cites W1987282211 @default.
• W2012711488 cites W1992677381 @default.
• W2012711488 cites W1997475045 @default.
• W2012711488 cites W1999517163 @default.
• W2012711488 cites W2001286327 @default.
• W2012711488 cites W2002024464 @default.
• W2012711488 cites W2006010807 @default.
• W2012711488 cites W2006604904 @default.
• W2012711488 cites W2006933991 @default.
• W2012711488 cites W2013118070 @default.
• W2012711488 cites W2015080610 @default.
• W2012711488 cites W2016388184 @default.
• W2012711488 cites W2017720816 @default.
• W2012711488 cites W2019204220 @default.
• W2012711488 cites W2024966103 @default.
• W2012711488 cites W2025548170 @default.
• W2012711488 cites W2029218412 @default.
• W2012711488 cites W2030756623 @default.
• W2012711488 cites W2034246647 @default.
• W2012711488 cites W2037595752 @default.
• W2012711488 cites W2037764579 @default.
• W2012711488 cites W2039669270 @default.
• W2012711488 cites W2046613022 @default.
• W2012711488 cites W2047691993 @default.
• W2012711488 cites W2048849036 @default.
• W2012711488 cites W2056680914 @default.
• W2012711488 cites W2062820647 @default.
• W2012711488 cites W2064489506 @default.
• W2012711488 cites W2074110416 @default.
• W2012711488 cites W2081197509 @default.
• W2012711488 cites W2082785827 @default.
• W2012711488 cites W2086848733 @default.
• W2012711488 cites W2094908580 @default.
• W2012711488 cites W2099166156 @default.
• W2012711488 cites W2114918609 @default.
• W2012711488 cites W2143711716 @default.
• W2012711488 cites W2148820585 @default.
• W2012711488 cites W2167812052 @default.
• W2012711488 cites W2170553025 @default.
• W2012711488 doi "https://doi.org/10.1002/jbm.a.31961" @default.
• W2012711488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18404706" @default.
• W2012711488 hasPublicationYear "2008" @default.
• W2012711488 type Work @default.
• W2012711488 sameAs 2012711488 @default.
• W2012711488 citedByCount "42" @default.
• W2012711488 countsByYear W20127114882012 @default.
• W2012711488 countsByYear W20127114882013 @default.
• W2012711488 countsByYear W20127114882014 @default.
• W2012711488 countsByYear W20127114882015 @default.
• W2012711488 countsByYear W20127114882016 @default.
• W2012711488 countsByYear W20127114882017 @default.
• W2012711488 countsByYear W20127114882019 @default.
• W2012711488 countsByYear W20127114882020 @default.
• W2012711488 countsByYear W20127114882022 @default.
• W2012711488 crossrefType "journal-article" @default.
• W2012711488 hasAuthorship W2012711488A5011916328 @default.
• W2012711488 hasAuthorship W2012711488A5017864062 @default.
• W2012711488 hasAuthorship W2012711488A5047609980 @default.
• W2012711488 hasAuthorship W2012711488A5050822671 @default.
• W2012711488 hasAuthorship W2012711488A5081601133 @default.
• W2012711488 hasConcept C104317684 @default.
• W2012711488 hasConcept C109316439 @default.
• W2012711488 hasConcept C121332964 @default.
• W2012711488 hasConcept C12554922 @default.
• W2012711488 hasConcept C135983454 @default.
• W2012711488 hasConcept C13965031 @default.
• W2012711488 hasConcept C142613039 @default.
• W2012711488 hasConcept C153356533 @default.
• W2012711488 hasConcept C161790260 @default.
• W2012711488 hasConcept C169274487 @default.
• W2012711488 hasConcept C178790620 @default.
• W2012711488 hasConcept C185592680 @default.
• W2012711488 hasConcept C188027245 @default.
• W2012711488 hasConcept C192562407 @default.
• W2012711488 hasConcept C202751555 @default.
• W2012711488 hasConcept C21951064 @default.
• W2012711488 hasConcept C2776573223 @default.
• W2012711488 hasConcept C2777516009 @default.
• W2012711488 hasConcept C2779732960 @default.
• W2012711488 hasConcept C521977710 @default.

• next