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• W2012747479 abstract "Electron spin resonance (ESR) spectroscopy in combination with site-directed spin labeling (SDSL) is a powerful tool for determining protein structure, dynamics and interactions. We report here a method for determining interactions between spin labels and paramagnetic relaxation agents, which is performed under subsaturating conditions. The low microwave-field amplitude employed (h1<0.36 G) only requires standard, commercially available ESR equipment. The effect of relaxation enhancement on the spin–spin-relaxation time, T2e, is measured by this method, and compared to classical progressive power saturation performed on a free spin label, (1-oxyl-2,2,5,5-tetramethyl-Δ3-pyrroline-3-methyl)methanethiosulfonate (MTSL), and a spin-labeled protein (Thermomyces lanuginosa lipase, TLL-I252C), employing the water-soluble relaxation agent chromium(III) oxalate (Crox) in concentrations between 0–10 mM. The low-amplitude theory showed excellent agreement with that of classical power saturation in quantifying Crox-induced relaxation enhancement. Low-amplitude measurements were then performed using a standard resonator, with Crox, on 11 spin-labeled TLL mutants displaying rotational correlation times in the motional narrowing regime. All spin-labeled proteins exhibited significant changes in T2e. We postulate that this novel method is especially suitable for studying moderately immobilized spin labels, such as those positioned at exposed sites in a protein. This method should prove useful for research groups with access to any ESR instrumentation." @default.
• W2012747479 created "2016-06-24" @default.
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• W2012747479 date "2004-08-01" @default.
• W2012747479 modified "2023-10-02" @default.
• W2012747479 title "Low microwave-amplitude ESR spectroscopy: Measuring spin-relaxation interactions of moderately immobilized spin labels in proteins" @default.
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• W2012747479 doi "https://doi.org/10.1016/j.jbbm.2004.05.002" @default.
• W2012747479 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15262447" @default.
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