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• W2012754005 abstract "Abstract Interspecific hybrid cells were isolated by fusion between thymidine kinase-deficient (TK − ) mouse B82 cells and hypoxanthine-guanine-phosphoribosyl-transferase-deficient (HGPRT − ) rat L6TG cells, and cultivating them in selective medium with hypoxanthine-aminopterin-thymidine (HAT). Karyo-type analysis revealed that they contained both mouse and rat chromosomes. Mitochondrial DNA (mtDNA) species of the hybrid cells were identified by digesting them with three kinds of restriction endonucleases, Hae II, EcoR I and Hpa II. Their restriction endonuclease cleavage patterns indicated that a portion of the mtDNAs was of mouse parent cell origin, while the remainings were recombinant molecules, i.e., part of the rat mtDNA sequence could be detected, but not whole rat mtDNA. The molecular weights of hybrid cell mtDNAs were calculated to be almost the same as that of the parent cells (~10 7 D)." @default.
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• W2012754005 date "1981-02-01" @default.
• W2012754005 modified "2023-10-18" @default.
• W2012754005 title "Analysis of mitochondrial DNA species in interspecific hybrid somatic cells using restriction endonucleases. Identification of recombinant mtDNA molecules" @default.
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• W2012754005 doi "https://doi.org/10.1016/0014-4827(81)90257-3" @default.
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