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• W2012779130 abstract "We have developed a method by which llama cytokine mRNAs can be quantified using real-time reverse transcription polymerase chain reaction (RT-PCR). Total RNA was extracted from lipopolysaccharide (LPS)-stimulated peripheral blood mononuclear cells (PBMCs) of llama, reverse transcribed to cDNA, and cytokine profiles for interleukin (IL)-1α, IL-1β, IL-6 and tumor necrosis factor (TNF) α were quantified by real-time PCR. The expressions of mRNAs of inflammatory cytokines IL-1α, IL-1β, IL-6 and TNFα were upregulated upon stimulation with LPS in a dose- and time-dependent manner. Incubation of PBMCs with 100 and 1,000 pg/ml of LPS for 3 to 6 hr resulted in the acceleration of the mRNA levels of inflammatory cytokines. Here, we describe a highly sensitive and reproducible method to quantify the transcription of llama cytokine mRNAs by real-time RT-PCR with the double-stranded DNA-binding dye SYBR Green I." @default.
• W2012779130 created "2016-06-24" @default.
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• W2012779130 date "2005-01-01" @default.
• W2012779130 modified "2023-10-14" @default.
• W2012779130 title "Quantification of Llama Inflammatory Cytokine mRNAs by Real-Time RT-PCR" @default.
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• W2012779130 doi "https://doi.org/10.1292/jvms.67.195" @default.
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