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• W2012807964 abstract "The genes encoding the 1,3-propanediol (1,3-PD) operon of Clostridium butyricum VPI1718 were characterized from a molecular and a biochemical point of view. This operon is composed of three genes, dhaB 1, dhaB 2, and dha T. When grown in a vitamin B12-free mineral medium with glycerol as carbon source, Escherichia coli expressing dhaB 1, dhaB 2, and dha T produces 1,3-PD and high glycerol dehydratase and 1,3-PD dehydrogenase activities. dhaB 1 and dhaB 2 encode, respectively, a new type of glycerol dehydratase and its activator protein. The deduced proteins DhaB1 and DhaB2, with calculated molecular masses of 88,074 and 34,149 Da, respectively, showed no homology with the known glycerol dehydratases that are all B12 dependent but significant similarity with the pyruvate formate lyases and pyruvate formate lyases activating enzymes and their homologues. The 1,158-bp dha T gene codes for a 1,3-PD dehydrogenase with a calculated molecular mass of 41,558 Da, revealing a high level of identity with other DhaT proteins from natural 1,3-PD producers. The expression of the 1,3-PD operon in C. butyricum is regulated at the transcriptional level, and this regulation seems to involve a two-component signal transduction system DhaAS/DhaA, which may have a similar function to DhaR, a transcriptional regulator found in other natural 1,3-PD producers. The discovery of a glycerol dehydratase, coenzyme B12 independent, should significantly influence the development of an economical vitamin B12-free biological process for the production of 1,3-PD from renewable resources." @default.
• W2012807964 created "2016-06-24" @default.
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• W2012807964 date "2003-04-18" @default.
• W2012807964 modified "2023-10-14" @default.
• W2012807964 title "Molecular characterization of the 1,3-propanediol (1,3-PD) operon of <i>Clostridium butyricum</i>" @default.
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• W2012807964 doi "https://doi.org/10.1073/pnas.0734105100" @default.
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