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- W2012809078 abstract "Abstract Thirty out of 40 sera from normal, nonimmunized rabbits reacted with smooth muscle in frozen sections of mouse stomach and with cytoplasmic filaments in cultured rat fibroblasts, mouse neuroblastoma, rat astrocytes, and rat Pt K2 epithelial cells. The immunofluorescent staining patterns seen in the cultured cells suggest that the fluorescent cytoplasmic structures are intermediate filaments. This suggestion was confirmed by the demonstration that all the activity of a high-titer, rabbit autoantibody was completely inhibited by serum absorption with skeletin, the intermediate filament protein from smooth muscle. Furthermore, the rabbit antibody reacted with the same structures in cultured cells as sera with defined antiintermediate filament or antiglial specificity. All but one of the rabbit autoantibodies were of low titer. The antibodies belonged to the IgG, IgM, or IgG and IgM immunoglobulin classes. Our observations show that smooth muscle autoantibodies occur frequently in sera from normal rabbits. These antibodies seem to recognize common antigenic determinants present in intermediate filaments from smooth muscle, fibroblasts, neuroblastoma, glial, and epithelial cells. The stimulus for rabbit autoantibody production is not known." @default.
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- W2012809078 date "1980-07-01" @default.
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- W2012809078 title "Smooth muscle autoantibodies reacting with cytoplasmic intermediate filaments in sera from normal, nonimmunized rabbits" @default.
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- W2012809078 doi "https://doi.org/10.1016/0090-1229(80)90133-6" @default.
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