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• W2012809290 abstract "Targeted sequence alteration would be an attractive method in gene therapy and biotechnology. To achieve in vivo targeted sequence alteration, a tailed duplex DNA consisting of annealed 35mer and 794mer single-stranded DNAs was delivered by means of hydrodynamic tail vein injection into liver of transgenic mouse harboring a reporter gene (the rpsL gene) in its genome. The tailed DNA was designed for a conversion of ATC to AGC at codon 80 of the rpsL transgene. The anticipated T-->G sequence alteration was induced in the transgene in the liver with an efficiency of approximately 0.1%. These results demonstrate the significant potential of this method for applications in gene therapy and biotechnology." @default.
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• W2012809290 date "2010-03-01" @default.
• W2012809290 modified "2023-10-14" @default.
• W2012809290 title "Targeted sequence alteration of a chromosomal locus in mouse liver" @default.
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• W2012809290 doi "https://doi.org/10.1016/j.ijpharm.2009.12.020" @default.
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