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• W2012818739 abstract "<h2>Abstract</h2> Natural products are an inexhaustible source for drug discovery. However, the validation and selection of primary screening assays are vital to guarantee a selection of extracts or molecules with relevant pharmacological action and worthy of following up. The assay must be rapid, simple, easy to implement, and produce quick results and preferably at a low cost. In this work, we developed and validated a colorimetric microtiter assay using the resazurin viability dye. The parameters of the resazurin method for high-throughput screening (HTS) using natural extracts against <i>Aspergillus fumigatus</i> were optimized and set up. The extracts plus RPMI-1640 modified medium containing the spores and 0.002% resazurin were added per well. The fluorescence was read after 24 to 30 h of incubation. The resazurin proved to be as suitable as Alamar Blue for determining the minimal inhibitory concentration of different antifungals against <i>A. fumigatus</i> and effective to analyze fungicidal and fungistatic compounds. An HTS of 12 000 microbial extracts was carried out against two <i>A. fumigatus</i> strains, and 2.7% of the extracts displayed antifungal activity. Our group has been the first to use this methodology for screening a collection of natural extracts to identify compounds with antifungal activity against the medically important human pathogen <i>A. fumigatus</i>." @default.
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• W2012818739 date "2012-04-01" @default.
• W2012818739 modified "2023-10-18" @default.
• W2012818739 title "A New Approach to Drug Discovery: High-Throughput Screening of Microbial Natural Extracts against Aspergillus fumigatus Using Resazurin" @default.
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• W2012818739 doi "https://doi.org/10.1177/1087057111433459" @default.
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