Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012841231> ?p ?o ?g. }
Showing items 1 to 92 of
92
with 100 items per page.
- W2012841231 endingPage "108" @default.
- W2012841231 startingPage "100" @default.
- W2012841231 abstract "PROBLEM: Sperm immobilizing antibodies present in sterile women may be one of the principal causes of immunological infertility. We already established cell lines that secrete recombinant human IgG sperm immobilizing antibody using class-switched (from IgM to IgG) genomic immunoglobulin genes. However, these transfectants produced a small quantity of antibody and required continuous use of a medium with selective reagents. We have now constructed cell lines that stably secrete the antibody in large quantities using immunoglobulin cDNAs and cDNA expression vectors. METHOD: The immunoglobulin heavy chain cDNA was cloned from transfectants that secrete the class-switched human IgG sperm immobilizing antibody. The light chain cDNA, which had already been cloned, and the heavy chain cDNA were inserted into the modified bovine papilloma virus-based cDNA expression vectors BCMGSNeo and BCMGSHyg, respectively. These constructs were sequentially transfected into a mouse myeloma cell line by electroporation. RESULTS: The established transfectants produced recombinant antibody that retained human sperm immobilizing activity in nonselective medium for at least 30 days. Moreover, the production of the antibody was increased three times over that of the previous cell lines. CONCLUSION: We have established an unique method that improves the production of sperm immobilizing antibody stably and in large quantities." @default.
- W2012841231 created "2016-06-24" @default.
- W2012841231 creator A5005150446 @default.
- W2012841231 creator A5007367111 @default.
- W2012841231 creator A5039083958 @default.
- W2012841231 creator A5053205447 @default.
- W2012841231 creator A5088404286 @default.
- W2012841231 date "1993-03-01" @default.
- W2012841231 modified "2023-10-03" @default.
- W2012841231 title "Stable Production of Recombinant Human Sperm Immobilizing Antibody Using cDNA Expression Vectors" @default.
- W2012841231 cites W1492717300 @default.
- W2012841231 cites W1533803295 @default.
- W2012841231 cites W1540461751 @default.
- W2012841231 cites W176145620 @default.
- W2012841231 cites W1970165143 @default.
- W2012841231 cites W1971301890 @default.
- W2012841231 cites W1973717772 @default.
- W2012841231 cites W1986132642 @default.
- W2012841231 cites W1988512414 @default.
- W2012841231 cites W1997257161 @default.
- W2012841231 cites W2005814055 @default.
- W2012841231 cites W2006643920 @default.
- W2012841231 cites W2011786643 @default.
- W2012841231 cites W2018107765 @default.
- W2012841231 cites W2022766921 @default.
- W2012841231 cites W2054592480 @default.
- W2012841231 cites W2054892173 @default.
- W2012841231 cites W2068817753 @default.
- W2012841231 cites W2099768712 @default.
- W2012841231 cites W2128056949 @default.
- W2012841231 cites W2138270253 @default.
- W2012841231 cites W2162399997 @default.
- W2012841231 doi "https://doi.org/10.1111/j.1600-0897.1993.tb00573.x" @default.
- W2012841231 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8329102" @default.
- W2012841231 hasPublicationYear "1993" @default.
- W2012841231 type Work @default.
- W2012841231 sameAs 2012841231 @default.
- W2012841231 citedByCount "2" @default.
- W2012841231 crossrefType "journal-article" @default.
- W2012841231 hasAuthorship W2012841231A5005150446 @default.
- W2012841231 hasAuthorship W2012841231A5007367111 @default.
- W2012841231 hasAuthorship W2012841231A5039083958 @default.
- W2012841231 hasAuthorship W2012841231A5053205447 @default.
- W2012841231 hasAuthorship W2012841231A5088404286 @default.
- W2012841231 hasConcept C104317684 @default.
- W2012841231 hasConcept C153911025 @default.
- W2012841231 hasConcept C159654299 @default.
- W2012841231 hasConcept C187882448 @default.
- W2012841231 hasConcept C203014093 @default.
- W2012841231 hasConcept C2781087480 @default.
- W2012841231 hasConcept C36394416 @default.
- W2012841231 hasConcept C40767141 @default.
- W2012841231 hasConcept C54009773 @default.
- W2012841231 hasConcept C54355233 @default.
- W2012841231 hasConcept C55493867 @default.
- W2012841231 hasConcept C81885089 @default.
- W2012841231 hasConcept C86803240 @default.
- W2012841231 hasConceptScore W2012841231C104317684 @default.
- W2012841231 hasConceptScore W2012841231C153911025 @default.
- W2012841231 hasConceptScore W2012841231C159654299 @default.
- W2012841231 hasConceptScore W2012841231C187882448 @default.
- W2012841231 hasConceptScore W2012841231C203014093 @default.
- W2012841231 hasConceptScore W2012841231C2781087480 @default.
- W2012841231 hasConceptScore W2012841231C36394416 @default.
- W2012841231 hasConceptScore W2012841231C40767141 @default.
- W2012841231 hasConceptScore W2012841231C54009773 @default.
- W2012841231 hasConceptScore W2012841231C54355233 @default.
- W2012841231 hasConceptScore W2012841231C55493867 @default.
- W2012841231 hasConceptScore W2012841231C81885089 @default.
- W2012841231 hasConceptScore W2012841231C86803240 @default.
- W2012841231 hasIssue "2" @default.
- W2012841231 hasLocation W20128412311 @default.
- W2012841231 hasLocation W20128412312 @default.
- W2012841231 hasOpenAccess W2012841231 @default.
- W2012841231 hasPrimaryLocation W20128412311 @default.
- W2012841231 hasRelatedWork W2338137066 @default.
- W2012841231 hasRelatedWork W2350146650 @default.
- W2012841231 hasRelatedWork W2352258017 @default.
- W2012841231 hasRelatedWork W2364319975 @default.
- W2012841231 hasRelatedWork W2367323167 @default.
- W2012841231 hasRelatedWork W2371820180 @default.
- W2012841231 hasRelatedWork W2386419125 @default.
- W2012841231 hasRelatedWork W2392841809 @default.
- W2012841231 hasRelatedWork W2394425474 @default.
- W2012841231 hasRelatedWork W2401891876 @default.
- W2012841231 hasVolume "29" @default.
- W2012841231 isParatext "false" @default.
- W2012841231 isRetracted "false" @default.
- W2012841231 magId "2012841231" @default.
- W2012841231 workType "article" @default.