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- W2012851891 abstract "An effective paradigm for transplanting large numbers of neural stem cells after central nervous system (CNS) injury has yet to be established. Biomaterial scaffolds have shown promise in cell transplantation and in regenerative medicine, but improved scaffolds are needed. In this study we designed and optimized multifunctional and biocompatible chitosan-based films and microspheres for the delivery of neural stem cells and growth factors for CNS injuries. The chitosan microspheres were fabricated by coaxial airflow techniques, with the sphere size controlled by varying the syringe needle gauge and the airflow rate. When applying a coaxial airflow at 30 standard cubic feet per hour, ∼300 μm diameter spheres were reproducibly generated that were physically stable yet susceptible to enzymatic degradation. Heparin was covalently crosslinked to the chitosan scaffolds using genipin, which bound fibroblast growth factor-2 (FGF-2) with high affinity while retaining its biological activity. At 1 μg ml−1 approximately 80% of the FGF-2 bound to the scaffold. A neural stem cell line, GFP + RG3.6 derived from embryonic rat cortex, was used to evaluate cytocompatibility, attachment and survival on the crosslinked chitosan–heparin complex surfaces. The MTT assay and microscopic analysis revealed that the scaffold containing tethered FGF-2 was superior in sustaining survival and growth of neural stem cells compared to standard culture conditions. Altogether, our results demonstrate that this multifunctional scaffold possesses good cytocompatibility and can be used as a growth factor delivery vehicle while supporting neural stem cell attachment and survival." @default.
- W2012851891 created "2016-06-24" @default.
- W2012851891 creator A5005833478 @default.
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- W2012851891 creator A5080919937 @default.
- W2012851891 date "2013-06-01" @default.
- W2012851891 modified "2023-10-17" @default.
- W2012851891 title "Heparin crosslinked chitosan microspheres for the delivery of neural stem cells and growth factors for central nervous system repair" @default.
- W2012851891 cites W1591817306 @default.
- W2012851891 cites W1971562703 @default.
- W2012851891 cites W1972979183 @default.
- W2012851891 cites W1974160158 @default.
- W2012851891 cites W1984734563 @default.
- W2012851891 cites W1988306902 @default.
- W2012851891 cites W1989910000 @default.
- W2012851891 cites W1992430132 @default.
- W2012851891 cites W1998771830 @default.
- W2012851891 cites W1999761643 @default.
- W2012851891 cites W2001337966 @default.
- W2012851891 cites W2001901378 @default.
- W2012851891 cites W2005966406 @default.
- W2012851891 cites W2007001763 @default.
- W2012851891 cites W2007731908 @default.
- W2012851891 cites W2008135428 @default.
- W2012851891 cites W2008204763 @default.
- W2012851891 cites W2008566143 @default.
- W2012851891 cites W2011440234 @default.
- W2012851891 cites W2013920156 @default.
- W2012851891 cites W2017174153 @default.
- W2012851891 cites W2018067227 @default.
- W2012851891 cites W2021771642 @default.
- W2012851891 cites W2022936165 @default.
- W2012851891 cites W2024250498 @default.
- W2012851891 cites W2026514183 @default.
- W2012851891 cites W2028340706 @default.
- W2012851891 cites W2029642897 @default.
- W2012851891 cites W2031493981 @default.
- W2012851891 cites W2033218391 @default.
- W2012851891 cites W2034829127 @default.
- W2012851891 cites W2035248906 @default.
- W2012851891 cites W2035338985 @default.
- W2012851891 cites W2038159444 @default.
- W2012851891 cites W2042110289 @default.
- W2012851891 cites W2042389213 @default.
- W2012851891 cites W2047885700 @default.
- W2012851891 cites W2048144165 @default.
- W2012851891 cites W2051371914 @default.
- W2012851891 cites W2054422937 @default.
- W2012851891 cites W2054992092 @default.
- W2012851891 cites W2060729643 @default.
- W2012851891 cites W2061077667 @default.
- W2012851891 cites W2064184046 @default.
- W2012851891 cites W2067727259 @default.
- W2012851891 cites W2071472660 @default.
- W2012851891 cites W2072529576 @default.
- W2012851891 cites W2073193426 @default.
- W2012851891 cites W2084671887 @default.
- W2012851891 cites W2085363859 @default.
- W2012851891 cites W2087193323 @default.
- W2012851891 cites W2088699248 @default.
- W2012851891 cites W2094847528 @default.
- W2012851891 cites W2094912630 @default.
- W2012851891 cites W2100859388 @default.
- W2012851891 cites W2101758592 @default.
- W2012851891 cites W2102557362 @default.
- W2012851891 cites W2104968232 @default.
- W2012851891 cites W2114918609 @default.
- W2012851891 cites W2116715149 @default.
- W2012851891 cites W2126486146 @default.
- W2012851891 cites W2134208433 @default.
- W2012851891 cites W2135652509 @default.
- W2012851891 cites W2135686778 @default.
- W2012851891 cites W2141033585 @default.
- W2012851891 cites W2145085027 @default.
- W2012851891 cites W2147326243 @default.
- W2012851891 cites W2148418279 @default.
- W2012851891 cites W2148450488 @default.
- W2012851891 cites W2158643446 @default.
- W2012851891 cites W2168583421 @default.
- W2012851891 cites W2330808969 @default.
- W2012851891 cites W23603308 @default.
- W2012851891 cites W4213109870 @default.
- W2012851891 cites W4237023775 @default.
- W2012851891 cites W4367435871 @default.
- W2012851891 doi "https://doi.org/10.1016/j.actbio.2013.02.043" @default.
- W2012851891 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23467042" @default.
- W2012851891 hasPublicationYear "2013" @default.
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