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• W2012900900 abstract "Human melanoma cells were examined in an indirect membrane immunofluorescence assay for surface nerve growth factor (NGF) and NGF receptors. This assay revealed that human melanoma cells have various levels of NGF and NGF receptors on the plasma membrane, whereas a variety of human sarcoma and carcinoma tumor cells and normal human fibroblasts are negative. Surface NGF could be detected on melanoma cells with a rabbit antiserum directed to NGF at titers as high as 1:64; prior adsorption of this antibody with mouse 2.5S NGF resulted in a loss of fluorescence. The melanoma cells were positive whether or not they were grown in the presence of fetal calf serum. NGF production by human melanomas is a previously unrecognized property of this differentiated cell type. Although other cells in culture have been shown to produce NGF, the association of NGF production with the presence of NGF receptors on the cell surface is rare among tumor cells, and may represent an opportunity for autostimulation of melanoma cells by this growth factor." @default.
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• W2012900900 date "1979-03-01" @default.
• W2012900900 modified "2023-09-24" @default.
• W2012900900 title "Human melanoma cells have both nerve growth factor and nerve growth factor-specific receptors on their cell surfaces." @default.
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• W2012900900 doi "https://doi.org/10.1073/pnas.76.3.1288" @default.
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