Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012912477> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W2012912477 endingPage "10261" @default.
- W2012912477 startingPage "10260" @default.
- W2012912477 abstract "An efficient mimic of the gp41 N-terminal coiled-coil trimer is described. The native protein mediates fusion of viral and cellular membranes, and its function is critical for infectivity. A central event in this process is formation of a “trimer of hairpins” structure in which a C-terminal gp41 sequence binds to a hydrophobic groove on the N-terminal trimer surface. Inhibition of this interaction is a promising therapeutic strategy, but the isolated trimer is not a convenient screening target, since the exposed hydrophobic pocket causes aggregation and precipitation. The problem has been circumvented in several ways such as attachment of auxiliary scaffolding elements or covalent subunit tethering. Here we report a more efficient approach, in which purely peptidic systems comparable in size to the native trimer display the expected specificity for the C-terminal ligand. Steric matching of 2:1 alanine/cyclohexylalanine core layers promotes formation of a 1:1:1 heterotrimer, whose surface interhelical interfaces can be uniquely controlled. Two of these interfaces contain solubilizing Glu/Lys pairs, while the third presents the gp41 interface. The model system binds the C-terminal peptide, while a control complex with only half the interface does not. A variety of biophysical methods are used to characterize the complex. The ability to control complex stoichiometry with only interior core residues should permit formation of any such interface, and extension to other viral systems is underway." @default.
- W2012912477 created "2016-06-24" @default.
- W2012912477 creator A5066862270 @default.
- W2012912477 creator A5078820044 @default.
- W2012912477 date "2004-08-01" @default.
- W2012912477 modified "2023-09-23" @default.
- W2012912477 title "Coiled-Coil Surface Presentation: An Efficient HIV gp41 Binding Interface Mimic" @default.
- W2012912477 cites W2038051119 @default.
- W2012912477 cites W2076016977 @default.
- W2012912477 cites W2092441129 @default.
- W2012912477 cites W2111491631 @default.
- W2012912477 cites W2125312330 @default.
- W2012912477 cites W2129160108 @default.
- W2012912477 cites W2143312620 @default.
- W2012912477 doi "https://doi.org/10.1021/ja047022w" @default.
- W2012912477 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/15315435" @default.
- W2012912477 hasPublicationYear "2004" @default.
- W2012912477 type Work @default.
- W2012912477 sameAs 2012912477 @default.
- W2012912477 citedByCount "5" @default.
- W2012912477 countsByYear W20129124772012 @default.
- W2012912477 countsByYear W20129124772016 @default.
- W2012912477 crossrefType "journal-article" @default.
- W2012912477 hasAuthorship W2012912477A5066862270 @default.
- W2012912477 hasAuthorship W2012912477A5078820044 @default.
- W2012912477 hasConcept C12554922 @default.
- W2012912477 hasConcept C147483822 @default.
- W2012912477 hasConcept C178790620 @default.
- W2012912477 hasConcept C185592680 @default.
- W2012912477 hasConcept C195616568 @default.
- W2012912477 hasConcept C2776258796 @default.
- W2012912477 hasConcept C2776403692 @default.
- W2012912477 hasConcept C2779546866 @default.
- W2012912477 hasConcept C54355233 @default.
- W2012912477 hasConcept C55493867 @default.
- W2012912477 hasConcept C55988834 @default.
- W2012912477 hasConcept C8010536 @default.
- W2012912477 hasConcept C86803240 @default.
- W2012912477 hasConceptScore W2012912477C12554922 @default.
- W2012912477 hasConceptScore W2012912477C147483822 @default.
- W2012912477 hasConceptScore W2012912477C178790620 @default.
- W2012912477 hasConceptScore W2012912477C185592680 @default.
- W2012912477 hasConceptScore W2012912477C195616568 @default.
- W2012912477 hasConceptScore W2012912477C2776258796 @default.
- W2012912477 hasConceptScore W2012912477C2776403692 @default.
- W2012912477 hasConceptScore W2012912477C2779546866 @default.
- W2012912477 hasConceptScore W2012912477C54355233 @default.
- W2012912477 hasConceptScore W2012912477C55493867 @default.
- W2012912477 hasConceptScore W2012912477C55988834 @default.
- W2012912477 hasConceptScore W2012912477C8010536 @default.
- W2012912477 hasConceptScore W2012912477C86803240 @default.
- W2012912477 hasIssue "33" @default.
- W2012912477 hasLocation W20129124771 @default.
- W2012912477 hasLocation W20129124772 @default.
- W2012912477 hasOpenAccess W2012912477 @default.
- W2012912477 hasPrimaryLocation W20129124771 @default.
- W2012912477 hasRelatedWork W1986025310 @default.
- W2012912477 hasRelatedWork W2003422665 @default.
- W2012912477 hasRelatedWork W2057330347 @default.
- W2012912477 hasRelatedWork W2087053830 @default.
- W2012912477 hasRelatedWork W2098923358 @default.
- W2012912477 hasRelatedWork W2111491631 @default.
- W2012912477 hasRelatedWork W2157585612 @default.
- W2012912477 hasRelatedWork W2559548168 @default.
- W2012912477 hasRelatedWork W2844344345 @default.
- W2012912477 hasRelatedWork W2991081874 @default.
- W2012912477 hasVolume "126" @default.
- W2012912477 isParatext "false" @default.
- W2012912477 isRetracted "false" @default.
- W2012912477 magId "2012912477" @default.
- W2012912477 workType "article" @default.