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- W2012912756 endingPage "e21860" @default.
- W2012912756 startingPage "e21860" @default.
- W2012912756 abstract "Mollusk shell formation is a fascinating aspect of biomineralization research. Shell matrix proteins play crucial roles in the control of calcium carbonate crystallization during shell formation in the pearl oyster, Pinctada fucata. Characterization of biomineralization-related genes during larval development could enhance our understanding of shell formation. Genes involved in shell biomineralization were isolated by constructing three suppression subtractive hybridization (SSH) libraries that represented genes expressed at key points during larval shell formation. A total of 2,923 ESTs from these libraries were sequenced and gave 990 unigenes. Unigenes coding for secreted proteins and proteins with tandem-arranged repeat units were screened in the three SSH libraries. A set of sequences coding for genes involved in shell formation was obtained. RT-PCR and in situ hybridization assays were carried out on five genes to investigate their spatial expression in several tissues, especially the mantle tissue. They all showed a different expression pattern from known biomineralization-related genes. Inhibition of the five genes by RNA interference resulted in different defects of the nacreous layer, indicating that they all were involved in aragonite crystallization. Intriguingly, one gene (UD_Cluster94.seq.Singlet1) was restricted to the 'aragonitic line'. The current data has yielded for the first time, to our knowledge, a suite of biomineralization-related genes active during the developmental stages of P. fucata, five of which were responsible for nacreous layer formation. This provides a useful starting point for isolating new genes involved in shell formation. The effects of genes on the formation of the 'aragonitic line', and other areas of the nacreous layer, suggests a different control mechanism for aragonite crystallization initiation from that of mature aragonite growth." @default.
- W2012912756 created "2016-06-24" @default.
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- W2012912756 creator A5060873221 @default.
- W2012912756 creator A5060955767 @default.
- W2012912756 date "2011-07-01" @default.
- W2012912756 modified "2023-10-10" @default.
- W2012912756 title "Identification of Genes Directly Involved in Shell Formation and Their Functions in Pearl Oyster, Pinctada fucata" @default.
- W2012912756 cites W1612420032 @default.
- W2012912756 cites W1665727945 @default.
- W2012912756 cites W1963700444 @default.
- W2012912756 cites W1966428938 @default.
- W2012912756 cites W1967052820 @default.
- W2012912756 cites W1968561160 @default.
- W2012912756 cites W1970831027 @default.
- W2012912756 cites W1973975677 @default.
- W2012912756 cites W197437642 @default.
- W2012912756 cites W1974671676 @default.
- W2012912756 cites W1981648501 @default.
- W2012912756 cites W1984360671 @default.
- W2012912756 cites W1985036864 @default.
- W2012912756 cites W1985484001 @default.
- W2012912756 cites W1987108879 @default.
- W2012912756 cites W1989025984 @default.
- W2012912756 cites W1991416066 @default.
- W2012912756 cites W1991731239 @default.
- W2012912756 cites W1995749067 @default.
- W2012912756 cites W1999869771 @default.
- W2012912756 cites W2004169264 @default.
- W2012912756 cites W2004468313 @default.
- W2012912756 cites W2005438107 @default.
- W2012912756 cites W2007318995 @default.
- W2012912756 cites W2008588240 @default.
- W2012912756 cites W2009091242 @default.
- W2012912756 cites W2010809008 @default.
- W2012912756 cites W2012077697 @default.
- W2012912756 cites W2013132563 @default.
- W2012912756 cites W2017952156 @default.
- W2012912756 cites W2019427380 @default.
- W2012912756 cites W2019455066 @default.
- W2012912756 cites W2020597515 @default.
- W2012912756 cites W2021032815 @default.
- W2012912756 cites W2022907134 @default.
- W2012912756 cites W2024350113 @default.
- W2012912756 cites W2024832968 @default.
- W2012912756 cites W2025809590 @default.
- W2012912756 cites W2026604651 @default.
- W2012912756 cites W2027674771 @default.
- W2012912756 cites W2029487298 @default.
- W2012912756 cites W2029805969 @default.
- W2012912756 cites W2031846886 @default.
- W2012912756 cites W2034911186 @default.
- W2012912756 cites W2037576312 @default.
- W2012912756 cites W2037951645 @default.
- W2012912756 cites W2040100879 @default.
- W2012912756 cites W2040849453 @default.
- W2012912756 cites W2048035576 @default.
- W2012912756 cites W2052959457 @default.
- W2012912756 cites W2063174088 @default.
- W2012912756 cites W2063341970 @default.
- W2012912756 cites W2063831343 @default.
- W2012912756 cites W2064650644 @default.
- W2012912756 cites W2065494287 @default.
- W2012912756 cites W2072972666 @default.
- W2012912756 cites W2074190431 @default.
- W2012912756 cites W2075286779 @default.
- W2012912756 cites W2078179425 @default.
- W2012912756 cites W2078395766 @default.
- W2012912756 cites W2078658097 @default.
- W2012912756 cites W2082923993 @default.
- W2012912756 cites W2089047063 @default.
- W2012912756 cites W2094438194 @default.
- W2012912756 cites W2095199125 @default.
- W2012912756 cites W2101972919 @default.
- W2012912756 cites W2102139220 @default.
- W2012912756 cites W2108230386 @default.
- W2012912756 cites W2118267499 @default.
- W2012912756 cites W2136845009 @default.
- W2012912756 cites W2138347099 @default.
- W2012912756 cites W2141605170 @default.
- W2012912756 cites W2152458080 @default.
- W2012912756 cites W2155143569 @default.
- W2012912756 cites W2155818894 @default.
- W2012912756 cites W2158192250 @default.
- W2012912756 cites W2164033494 @default.
- W2012912756 cites W2164154943 @default.
- W2012912756 cites W2165463056 @default.
- W2012912756 cites W2171075112 @default.
- W2012912756 cites W2171091522 @default.
- W2012912756 cites W4239858085 @default.
- W2012912756 doi "https://doi.org/10.1371/journal.pone.0021860" @default.
- W2012912756 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/3128620" @default.
- W2012912756 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21747964" @default.
- W2012912756 hasPublicationYear "2011" @default.
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