FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012914327> ?p ?o ?g. }

• W2012914327 endingPage "1416" @default.
• W2012914327 startingPage "1413" @default.
• W2012914327 abstract "Abstract Changes in the intrinsic protein fluorescence with the additive concentration provide one of the most employed methodologies for the evaluation of the binding constant and the number of binding sites. In the last years, more than 175 studies have been published where the double logarithmic plot shown below is used toward determining the number of equivalent binding sites ( n ). Log [( F ° − F ) / F ] = log K + n log [Q 0 ]. However, the value of n evaluated by this procedure is unrelated to the number of equivalent binding sites; rather it represents the stoichiometry of the binding step. The confusion on the meaning of n arises upon assuming that the binding process is represented by the forward and backward elementary steps shown below, implying that binding of the n solutes takes place simultaneously, i.e. there are no intermediate species. n Q + P ⇆ Q n P. The conclusion that n is unrelated to the number of equivalent binding sites is supported by the fact that in all the systems considered (99% of them) n values are close to one and much smaller than those obtained by ultrafiltration. It is then remarkable, the profusion of publications in peer‐reviewed, specialized journals including a conceptual error that confuses Hill′s coefficient and/or the stoichiometry of the binding step with the number of independent binding sites. Here, we discuss the origin of this common misconception and provide alternative methods to determine the number of binding sites." @default.
• W2012914327 created "2016-06-24" @default.
• W2012914327 creator A5028732265 @default.
• W2012914327 creator A5059830625 @default.
• W2012914327 creator A5084124414 @default.
• W2012914327 date "2013-08-26" @default.
• W2012914327 modified "2023-10-16" @default.
• W2012914327 title "Evaluation of the Number of Binding Sites in Proteins from their Intrinsic Fluorescence: Limitations and Pitfalls" @default.
• W2012914327 cites W1513938578 @default.
• W2012914327 cites W1540977682 @default.
• W2012914327 cites W1586978943 @default.
• W2012914327 cites W1963661719 @default.
• W2012914327 cites W1966024282 @default.
• W2012914327 cites W1966145381 @default.
• W2012914327 cites W1968716120 @default.
• W2012914327 cites W1974190162 @default.
• W2012914327 cites W1980457952 @default.
• W2012914327 cites W1980999798 @default.
• W2012914327 cites W1994501205 @default.
• W2012914327 cites W1994930430 @default.
• W2012914327 cites W1999796991 @default.
• W2012914327 cites W2001770439 @default.
• W2012914327 cites W2004035410 @default.
• W2012914327 cites W2004458222 @default.
• W2012914327 cites W2005755902 @default.
• W2012914327 cites W2008166117 @default.
• W2012914327 cites W2008505934 @default.
• W2012914327 cites W2008866993 @default.
• W2012914327 cites W2011217357 @default.
• W2012914327 cites W2012481624 @default.
• W2012914327 cites W2013998513 @default.
• W2012914327 cites W2022788669 @default.
• W2012914327 cites W2028687473 @default.
• W2012914327 cites W2028689547 @default.
• W2012914327 cites W2038139808 @default.
• W2012914327 cites W2043738471 @default.
• W2012914327 cites W2045111137 @default.
• W2012914327 cites W2045907813 @default.
• W2012914327 cites W2048552262 @default.
• W2012914327 cites W2053546502 @default.
• W2012914327 cites W2053637769 @default.
• W2012914327 cites W2054102893 @default.
• W2012914327 cites W2056274562 @default.
• W2012914327 cites W2062940078 @default.
• W2012914327 cites W2063790506 @default.
• W2012914327 cites W2068226693 @default.
• W2012914327 cites W2075017779 @default.
• W2012914327 cites W2075424134 @default.
• W2012914327 cites W2075534283 @default.
• W2012914327 cites W2080480885 @default.
• W2012914327 cites W2080494649 @default.
• W2012914327 cites W2080640262 @default.
• W2012914327 cites W2082645388 @default.
• W2012914327 cites W2089797648 @default.
• W2012914327 cites W2092409587 @default.
• W2012914327 cites W2095438882 @default.
• W2012914327 cites W2114195734 @default.
• W2012914327 cites W2114761249 @default.
• W2012914327 cites W2121303050 @default.
• W2012914327 cites W2163706749 @default.
• W2012914327 doi "https://doi.org/10.1111/php.12112" @default.
• W2012914327 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/23789593" @default.
• W2012914327 hasPublicationYear "2013" @default.
• W2012914327 type Work @default.
• W2012914327 sameAs 2012914327 @default.
• W2012914327 citedByCount "35" @default.
• W2012914327 countsByYear W20129143272014 @default.
• W2012914327 countsByYear W20129143272015 @default.
• W2012914327 countsByYear W20129143272016 @default.
• W2012914327 countsByYear W20129143272017 @default.
• W2012914327 countsByYear W20129143272018 @default.
• W2012914327 countsByYear W20129143272019 @default.
• W2012914327 countsByYear W20129143272020 @default.
• W2012914327 countsByYear W20129143272021 @default.
• W2012914327 countsByYear W20129143272022 @default.
• W2012914327 countsByYear W20129143272023 @default.
• W2012914327 crossrefType "journal-article" @default.
• W2012914327 hasAuthorship W2012914327A5028732265 @default.
• W2012914327 hasAuthorship W2012914327A5059830625 @default.
• W2012914327 hasAuthorship W2012914327A5084124414 @default.
• W2012914327 hasConcept C107824862 @default.
• W2012914327 hasConcept C11171543 @default.
• W2012914327 hasConcept C134306372 @default.
• W2012914327 hasConcept C144082473 @default.
• W2012914327 hasConcept C147789679 @default.
• W2012914327 hasConcept C15744967 @default.
• W2012914327 hasConcept C185592680 @default.
• W2012914327 hasConcept C2781140086 @default.
• W2012914327 hasConcept C32786932 @default.
• W2012914327 hasConcept C33923547 @default.
• W2012914327 hasConcept C39927690 @default.
• W2012914327 hasConcept C55493867 @default.
• W2012914327 hasConceptScore W2012914327C107824862 @default.
• W2012914327 hasConceptScore W2012914327C11171543 @default.
• W2012914327 hasConceptScore W2012914327C134306372 @default.
• W2012914327 hasConceptScore W2012914327C144082473 @default.
• W2012914327 hasConceptScore W2012914327C147789679 @default.
• W2012914327 hasConceptScore W2012914327C15744967 @default.

• next