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• W2012930721 abstract "No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Aug 2001REGULATION OF KERATINOCYTE GROWTH FACTOR RECEPTOR AND ANDROGEN RECEPTOR IN EPITHELIAL CELLS OF THE HUMAN PROSTATE BERNHARD PLANZ, QIFA WANG, SANDRA D. KIRLEY, MICHAEL MARBERGER, and W. SCOTT MCDOUGAL BERNHARD PLANZBERNHARD PLANZ More articles by this author , QIFA WANGQIFA WANG More articles by this author , SANDRA D. KIRLEYSANDRA D. KIRLEY More articles by this author , MICHAEL MARBERGERMICHAEL MARBERGER More articles by this author , and W. SCOTT MCDOUGALW. SCOTT MCDOUGAL More articles by this author View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)66042-9AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: Stromal-epithelial interactions of growth factors and the androgen receptor may have implications for the pathophysiology of benign and neoplastic transformation of the human adult prostate. We investigated a possible interaction of keratinocyte growth factor with its receptor as well as with the androgen receptor signaling pathway in human prostatic epithelial cells. Materials and Methods: Human prostatic epithelial cells were obtained from explant primary culture, established in DU145 cell conditioned medium and maintained in keratinocyte serum-free medium with supplements. Epithelial cells were characterized by light and electron microscopy, and immunocytochemical study using epithelial and mesenchymal markers. Androgen receptor, keratinocyte growth factor receptor and keratinocyte growth factor messenger RNA expression was measured by polymerase chain reaction (PCR). The response to 0.01 to 10 nM. dihydrotestosterone, 10 μM. flutamide and 1 to 1,000 ng./ml. keratinocyte growth factor was tested by [3H] thymidine assay. The difference in keratinocyte growth factor receptor and androgen receptor gene expression after treatment with and without keratinocyte growth factor and flutamide were determined by quantitative multiplex PCR and quantitated using densitometry analysis. Results: Immunocytochemical and electron microscopy characterization revealed typical epithelial differentiation. PCR showed keratinocyte growth factor receptor and androgen receptor expression in epithelial cultured cells but no keratinocyte growth factor expression. Epithelial cells showed a significant time and dose dependent stimulation of cell proliferation with keratinocyte growth factor and dihydrotestosterone (p <0.05). When combined with the anti-androgen flutamide the effect of 100 ng./ml. keratinocyte growth factor was significantly decreased (p <0.05). At 100 ng./ml. keratinocyte growth factor quantitative multiplex PCR revealed stimulated keratinocyte growth factor receptor and androgen receptor messenger RNA expression. Conclusions: These results show that keratinocyte growth factor up-regulates the keratinocyte growth factor and androgen receptors in the absence of androgen. Thus, the androgen signaling pathway may be activated by growth factors such as keratinocyte growth factor in an androgen deficient environment. References 1 : Steroid hormone receptors: many actors in search of a plot. Cell1995; 83: 851. Google Scholar 2 : Characterization and expression of a cDNA encoding the human androgen receptor. Proc Natl Acad Sci USA1989; 86: 327. Google Scholar 3 : Immunolocalization of the keratinocyte growth factor in benign and neoplastic human prostate and its relation to androgen receptor. Prostate1999; 41: 233. Google Scholar 4 : Androgen receptor gene and hormonal therapy failure of prostate cancer. Am J Pathol1998; 152: 1. 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Google Scholar From the Department of Urology, University of Vienna, Vienna, Austria, and Departments of Urology and Reproductive Endocrinology, Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts© 2001 by American Urological Association, Inc.FiguresReferencesRelatedDetails Volume 166Issue 2August 2001Page: 678-683 Advertisement Copyright & Permissions© 2001 by American Urological Association, Inc.Keywordsprostategrowth factor receptorsreceptorsandrogenepitheliumMetricsAuthor Information BERNHARD PLANZ More articles by this author QIFA WANG More articles by this author SANDRA D. KIRLEY More articles by this author MICHAEL MARBERGER More articles by this author W. SCOTT MCDOUGAL More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
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