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- W2012946510 abstract "Oxidation and lipogenesis in isolated rat lung tissue were studied in vitro. The minced tissue was incubated in a Krebs-Ringer bicarbonate buffer (pH 7.4) with 1-14C-acetate, 2-14C-pyruvate, U-14C-D-glucose, 1,5-14C-citrate, 1-14C-laurate, 1-14C-palmitate, 1-14C-stearate, 1-14C-oleate, 1-14C-linoleate. The lung tissue readily oxidized all of these substrates to14CO2 and incorporated them into14C-lipids with the exception of 1,5-14C-citrate, for which there was no significant incorporation into14C-lipids. Most of the lipid14C was recovered in phospholipids, more specifically phosphatidyl choline. Twenty-eight per cent of glucose carbons was incorporated into the fatty acid moiety of phospholipids, while more than 90% of the carbons of other substrates was found in phospholipid fatty acids. The main fatty acid of the phospholipid fraction synthesized from acetate, pyruvate or glucose was palmitic acid. The oxidation of fatty acids was apparently influenced both by the carbon chain length and number of double bonds. Accumulation of14C-fatty acids in the tissue was observed when fatty acids were used as substrates; this finding suggests that the rate limiting step was not in the uptake of fatty acids. Chemical degradation of14C-myristic and palmitic acids obtained by hydrolysis of phospholipids biosynthesized from 1-14C-laurate indicated that the phospholipid fatty acids were synthesized via the de novo synthesis pathway." @default.
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- W2012946510 title "Lipid synthesis by rat lung in vitro" @default.
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- W2012946510 doi "https://doi.org/10.1007/bf02533065" @default.
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