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- W2012969931 abstract "Summary Cotyledonary protoplasts of two French fibre flax ( Linum usitatissimum ) cultivars (Ariane and Viking) were isolated and cultured. The optimal conditions for high yield were from 4-5 day old seedlings, where 6-6.5x10 6 and 6.7-7.4x10 6 protoplasts were released from cv. Ariane and cv. Viking respectively. Culture in glutamine (20mM) containing medium led to first divisions after 2 days with an efficiency of 19% or 21.9% (cv. Ariane) and 49.3% or 44% (cv. Viking) of divided cells, when plating densities were 10 5 versus 5 x 10 4 protoplasts per mL. Rhizogenesis occurred on protoplast derived calli at high frequency 4 weeks after transfer onto solid medium supplemented with 0.5 μM 2.4 dichlorophenoxyacetic acid. Variations of cell wall sugar composition during culture on inducing and non-inducing media expressed some significant differences. The percentage of galacturonic acid increased on non-inducing medium, but remained constant on root-inducing medium. Glucose presented a peak of accumulation concomitant of the emergence of the root meristems 4 weeks after transfer onto 2,4-D containing medium. On the contrary galactose and arabinose variations were not characteristic of the rhizogenic process." @default.
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- W2012969931 date "1994-03-01" @default.
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- W2012969931 title "Cell Wall Composition and Morphogenic Response in Callus Derived from Protoplasts of two Fibre flax (Linum usitatissimum L.) Genotypes" @default.
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