Matches in SemOpenAlex for { <https://semopenalex.org/work/W2012979946> ?p ?o ?g. }
- W2012979946 endingPage "175" @default.
- W2012979946 startingPage "166" @default.
- W2012979946 abstract "The A/Victoria/3/75 (H3N2‐subtype ) hemagglutinin ( HA ) gene was engineered for expression in Pichia pastoris as a soluble secreted molecule. The HA cDNA lacking the C‐terminal transmembrane anchor‐coding sequence was fused to the Saccharomyces cerevisiae α‐mating factor secretion signal and placed under control of the methanol‐inducible P. pastoris alcohol oxidase 1 (AOX1) promoter. Growth of transformants on methanol‐containing medium resulted in the secretion of recombinant non‐cleaved soluble hemagglutinin (HA0s). Remarkably, the pH of the induction medium had an important effect on the expression level, the highest level being obtained at pH 8.0. The gel filtration profile and the reactivity against a panel of different HA‐conformation specific monoclonal antibodies indicated that HA0s was monomeric. Analysis of the N‐linked glycans revealed a typical P. pastoris type of glycosylation, consisting of glycans with 10–12 glycosyl residues. Mice immunized with purified soluble hemagglutinin (HA0s) showed complete protection against a challenge with 10 LD 50 of mouse‐adapted homologous virus (X47), whereas all control mice succumbed. Heterologous challenge with X31 virus [A/Aichi/2/68 (H3N2‐subtype)], resulted in significantly higher survival rates in the immunized group compared with the control group. These results, together with the safety, reliability and economic potential of P. pastoris , as well as the flexibility and fast adaptation of the expression system may allow development of an effective recombinant influenza vaccine." @default.
- W2012979946 created "2016-06-24" @default.
- W2012979946 creator A5016148840 @default.
- W2012979946 creator A5027231686 @default.
- W2012979946 creator A5043395687 @default.
- W2012979946 creator A5047888158 @default.
- W2012979946 creator A5064663075 @default.
- W2012979946 creator A5070224625 @default.
- W2012979946 creator A5076009877 @default.
- W2012979946 creator A5083518782 @default.
- W2012979946 date "1999-02-05" @default.
- W2012979946 modified "2023-10-17" @default.
- W2012979946 title "Protection of mice against a lethal influenza virus challenge after immunization with yeast-derived secreted influenza virus hemagglutinin" @default.
- W2012979946 cites W145154849 @default.
- W2012979946 cites W1550184056 @default.
- W2012979946 cites W1594347825 @default.
- W2012979946 cites W1607018086 @default.
- W2012979946 cites W1655175605 @default.
- W2012979946 cites W1853564911 @default.
- W2012979946 cites W1966690722 @default.
- W2012979946 cites W1971312572 @default.
- W2012979946 cites W1971371254 @default.
- W2012979946 cites W1974048907 @default.
- W2012979946 cites W1974517984 @default.
- W2012979946 cites W1974587421 @default.
- W2012979946 cites W1979537384 @default.
- W2012979946 cites W1980823976 @default.
- W2012979946 cites W1987042667 @default.
- W2012979946 cites W1991978326 @default.
- W2012979946 cites W1998285838 @default.
- W2012979946 cites W2001675439 @default.
- W2012979946 cites W200246087 @default.
- W2012979946 cites W2004402674 @default.
- W2012979946 cites W2005203390 @default.
- W2012979946 cites W2008765994 @default.
- W2012979946 cites W2013453931 @default.
- W2012979946 cites W2018529206 @default.
- W2012979946 cites W2024999105 @default.
- W2012979946 cites W2029230587 @default.
- W2012979946 cites W2033658186 @default.
- W2012979946 cites W2042611040 @default.
- W2012979946 cites W2044304655 @default.
- W2012979946 cites W2044952215 @default.
- W2012979946 cites W2047054663 @default.
- W2012979946 cites W2050871684 @default.
- W2012979946 cites W2059859060 @default.
- W2012979946 cites W2061872330 @default.
- W2012979946 cites W2062207296 @default.
- W2012979946 cites W2063554961 @default.
- W2012979946 cites W2064805716 @default.
- W2012979946 cites W2070426326 @default.
- W2012979946 cites W2073459612 @default.
- W2012979946 cites W2083963178 @default.
- W2012979946 cites W2092846883 @default.
- W2012979946 cites W2107663572 @default.
- W2012979946 cites W2114711026 @default.
- W2012979946 cites W2119460480 @default.
- W2012979946 cites W2131937710 @default.
- W2012979946 cites W2138176875 @default.
- W2012979946 cites W2141492848 @default.
- W2012979946 cites W2154116964 @default.
- W2012979946 cites W2157100325 @default.
- W2012979946 cites W2163960578 @default.
- W2012979946 cites W2225237934 @default.
- W2012979946 cites W41359786 @default.
- W2012979946 cites W4292199690 @default.
- W2012979946 cites W4293247451 @default.
- W2012979946 doi "https://doi.org/10.1046/j.1432-1327.1999.00150.x" @default.
- W2012979946 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10091596" @default.
- W2012979946 hasPublicationYear "1999" @default.
- W2012979946 type Work @default.
- W2012979946 sameAs 2012979946 @default.
- W2012979946 citedByCount "59" @default.
- W2012979946 countsByYear W20129799462012 @default.
- W2012979946 countsByYear W20129799462013 @default.
- W2012979946 countsByYear W20129799462014 @default.
- W2012979946 countsByYear W20129799462015 @default.
- W2012979946 countsByYear W20129799462016 @default.
- W2012979946 countsByYear W20129799462019 @default.
- W2012979946 countsByYear W20129799462020 @default.
- W2012979946 countsByYear W20129799462021 @default.
- W2012979946 crossrefType "journal-article" @default.
- W2012979946 hasAuthorship W2012979946A5016148840 @default.
- W2012979946 hasAuthorship W2012979946A5027231686 @default.
- W2012979946 hasAuthorship W2012979946A5043395687 @default.
- W2012979946 hasAuthorship W2012979946A5047888158 @default.
- W2012979946 hasAuthorship W2012979946A5064663075 @default.
- W2012979946 hasAuthorship W2012979946A5070224625 @default.
- W2012979946 hasAuthorship W2012979946A5076009877 @default.
- W2012979946 hasAuthorship W2012979946A5083518782 @default.
- W2012979946 hasBestOaLocation W20129799461 @default.
- W2012979946 hasConcept C104317684 @default.
- W2012979946 hasConcept C153911025 @default.
- W2012979946 hasConcept C159047783 @default.
- W2012979946 hasConcept C2522874641 @default.
- W2012979946 hasConcept C2777546802 @default.
- W2012979946 hasConcept C2778692840 @default.
- W2012979946 hasConcept C2779732469 @default.