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- W2012998889 abstract "Abstract A method to determine lactose in dairy products by high-performance liquid chromatography is described. Samples suitable for assay were prepared from the liquid state by deproteinization with trichloroacetic acid (6% wt/vol, final concentration). Lactose was separated by injection of the deproteinized sample onto a μ Bondapak/Carbohydrate® 3 3Waters Associates, Milford, MA. column and isocratic elution with an acetonitrile/water mobile phase. Detection was with a differential refractometer. Sequential injection of a lactose standard and sample, addition of a standard quantity of lactose to the sample, and addition of an internal standard (xylose) to the sample were techniques for quantitation. The validity of high-performance liquid chromatography as a method for determining lactose concentration in milk products was supported by recovery experiments and comparison of results with those by a phenol-sulfuric acid, colorimetric assay." @default.
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- W2012998889 modified "2023-09-26" @default.
- W2012998889 title "Determination of Lactose in Milk Products by High-Performance Liquid Chromatography" @default.
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- W2012998889 doi "https://doi.org/10.3168/jds.s0022-0302(79)83310-x" @default.
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