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• W2013005248 abstract "Aerobic photo-bleaching of carotenoids in isolated spinach chloroplasts was investigated by measuring the absorption spectrum of chloroplasts and by analysing carotenoid components by means of thin-layer chromatography on microcrystalline cellulose. Carotenoids were irreversibly bleached when chloroplasts were illuminated by red light in the presence of ferricyanide or an inhibitor of the Hill reaction such as azide, hydroxylamine and carbonylcyanide m-chlorophenylhydrazone, while carotenoids in the chloroplasts suspended in a phosphate buffer in the absence of these agents underwent no bleaching on illumination. The photo-bleaching of carotenoids in the presence of these agents was suppressed by another group of inhibitors of the Hill reaction such as o-phenanthroline, substituted phenylureas and symmetrical triazines, or by reducing agents such as ascorbate and ascorbate plusN,N,N′,N′-tetramethyl-p-phenylenediamine. Chromatographic analysis of carotenoids extracted from the chloroplasts illuminated in the presence of carbonylcyanide m-chlorophenyl-hydrazone or ferricyanide showed that three major carotenoid components, carotenes, lutein and violaxanthin, were bleached to similar and considerable extents. Neoxanthin was much more resistant to the action of light. The photo-bleaching of lutein and violaxanthin proceeded more rapidly in air than in a nitrogen atmosphere, but the bleaching of carotenes proceeded at an equal rate under these conditions. These results are discussed in relation to the photochemical electron transport in chloroplasts." @default.
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• W2013005248 date "1969-04-01" @default.
• W2013005248 modified "2023-10-16" @default.
• W2013005248 title "Photo-bleaching of carotenoids related to the electron transport in chloroplasts" @default.
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• W2013005248 doi "https://doi.org/10.1016/0005-2728(69)90147-9" @default.
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