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• W2013012468 endingPage "559" @default.
• W2013012468 startingPage "539" @default.
• W2013012468 abstract "A number of basic features of the infrared spectrum of keratin have been confirmed and some new features have been found. In the 3-μ region, the amide A frequency of helical material in α-keratin at 3286 cm.−1 is close to the expected value, but that of the crystalline phase in α-keratin, near 3270 cm.−1, is lower than had previously been reported. The noncrystalline phase absorbs in the vicinity of 3300 cm.−1 or above, and this causes the low-intensity component of the amide A band in both α- and β-keratin to occur at higher frequencies than those of the high-intensity component. In the 6-μ region, the amide II frequency of noncrystalline material is below 1525 cm.−1. Keratin denatured in lithium bromide, after washing out the reagent, appears to have a considerable helix content, possibly as much as that of the original protein. Hydration causes significant spectral changes. In the 6-mu; region, the frequency of the amide I band of crystalline material is lowered, while that of the amide II band is increased, both by a few wavenumbers; the amide II frequency of noncrystaline material is also increased by a few wavenumbers. In the 3-μ region, no significant change is observed in the amide A frequency of crystalline material, while the frequency of the noncrystaline material is reduced. These spectral changes are interpreted in terms of a weak association of water with main-chain carbonyl groups in the crystalline phase, while in the noncrystaline phase it is thought likely that water molecules form hydrogen-bond bridges between polypetide chains. The absorption coefficient of the amide A band and the integrated absorption intensities of the amide A, I, and II bands do not vary appreciably in the three forms of keratin investigated." @default.
• W2013012468 created "2016-06-24" @default.
• W2013012468 creator A5027276102 @default.
• W2013012468 date "1966-05-01" @default.
• W2013012468 modified "2023-10-13" @default.
• W2013012468 title "Infrared absorption spectrum of keratin. I. Spectra of ?-, ?-, and supercontracted keratin" @default.
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• W2013012468 doi "https://doi.org/10.1002/bip.1966.360040506" @default.
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