Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013022634> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W2013022634 endingPage "716" @default.
- W2013022634 startingPage "709" @default.
- W2013022634 abstract "A cDNA for cytochrome b(5) was cloned from a cDNA library of buds of the ascidian, Polyandrocarpa misakiensis, by a hybridization method involving a digoxigenin-labeled cDNA probe of human soluble cytochrome b(5). The nucleotide sequence of the cDNA for the ascidian cytochrome b(5) (Pmb5) consisted of about 1,800 base pairs including 5'- and 3'-noncoding regions, and a coding sequence of 405 base pairs. The amino acid sequence of 135 residues deduced from the coding nucleotide sequence exhibited 54% identity and 76% similarity to chicken cytochrome b(5). A highly conserved amino acid sequence was observed in the amino-terminal domain of 96 residues containing two heme-binding histidine residues. The putative soluble form of the recombinant Pmb5 expressed in Escherichia coli was purified to homogeneity by column chromatographies on an anion-exchanger and gel filtration. The purified Pmb5 showed the typical absorption spectrum of cytochrome b(5) with an asymmetric peak at 556 nm and a shoulder at 560 nm upon reduction with NADH and NADH-cytochrome b(5) reductase. The low temperature spectrum of the dithionite-reduced form of the protein contained the split peaks at 551 and 555 nm, this spectrum being very similar to that of mammalian liver cytochrome b(5). Expression of Pmb5 in the ascidian was examined immunohistochemically with a monoclonal antibody against the Pmb5. Apparently high level expression of Pmb5 was found in the developing buds, but the levels of cytochrome b(5) in the parents and juvenile adults were very low. This is the first report on the characterization of Pmb5, and the increased expression of Pmb5 in the ascidian." @default.
- W2013022634 created "2016-06-24" @default.
- W2013022634 creator A5013791617 @default.
- W2013022634 creator A5024919584 @default.
- W2013022634 creator A5034537316 @default.
- W2013022634 creator A5037246648 @default.
- W2013022634 creator A5045247574 @default.
- W2013022634 date "2001-05-01" @default.
- W2013022634 modified "2023-09-27" @default.
- W2013022634 title "Characterization of Cytochrome b5 in the Ascidian Polyandrocarpa misakiensis and Budding-Specific Expression" @default.
- W2013022634 doi "https://doi.org/10.1093/oxfordjournals.jbchem.a002910" @default.
- W2013022634 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11328592" @default.
- W2013022634 hasPublicationYear "2001" @default.
- W2013022634 type Work @default.
- W2013022634 sameAs 2013022634 @default.
- W2013022634 citedByCount "3" @default.
- W2013022634 crossrefType "journal-article" @default.
- W2013022634 hasAuthorship W2013022634A5013791617 @default.
- W2013022634 hasAuthorship W2013022634A5024919584 @default.
- W2013022634 hasAuthorship W2013022634A5034537316 @default.
- W2013022634 hasAuthorship W2013022634A5037246648 @default.
- W2013022634 hasAuthorship W2013022634A5045247574 @default.
- W2013022634 hasConcept C104317684 @default.
- W2013022634 hasConcept C133166286 @default.
- W2013022634 hasConcept C148292235 @default.
- W2013022634 hasConcept C153911025 @default.
- W2013022634 hasConcept C167625842 @default.
- W2013022634 hasConcept C181199279 @default.
- W2013022634 hasConcept C187882448 @default.
- W2013022634 hasConcept C24586158 @default.
- W2013022634 hasConcept C2776217839 @default.
- W2013022634 hasConcept C2778460671 @default.
- W2013022634 hasConcept C2780768313 @default.
- W2013022634 hasConcept C28859421 @default.
- W2013022634 hasConcept C29311851 @default.
- W2013022634 hasConcept C515207424 @default.
- W2013022634 hasConcept C55493867 @default.
- W2013022634 hasConcept C84148353 @default.
- W2013022634 hasConcept C86803240 @default.
- W2013022634 hasConceptScore W2013022634C104317684 @default.
- W2013022634 hasConceptScore W2013022634C133166286 @default.
- W2013022634 hasConceptScore W2013022634C148292235 @default.
- W2013022634 hasConceptScore W2013022634C153911025 @default.
- W2013022634 hasConceptScore W2013022634C167625842 @default.
- W2013022634 hasConceptScore W2013022634C181199279 @default.
- W2013022634 hasConceptScore W2013022634C187882448 @default.
- W2013022634 hasConceptScore W2013022634C24586158 @default.
- W2013022634 hasConceptScore W2013022634C2776217839 @default.
- W2013022634 hasConceptScore W2013022634C2778460671 @default.
- W2013022634 hasConceptScore W2013022634C2780768313 @default.
- W2013022634 hasConceptScore W2013022634C28859421 @default.
- W2013022634 hasConceptScore W2013022634C29311851 @default.
- W2013022634 hasConceptScore W2013022634C515207424 @default.
- W2013022634 hasConceptScore W2013022634C55493867 @default.
- W2013022634 hasConceptScore W2013022634C84148353 @default.
- W2013022634 hasConceptScore W2013022634C86803240 @default.
- W2013022634 hasIssue "5" @default.
- W2013022634 hasLocation W20130226341 @default.
- W2013022634 hasLocation W20130226342 @default.
- W2013022634 hasOpenAccess W2013022634 @default.
- W2013022634 hasPrimaryLocation W20130226341 @default.
- W2013022634 hasRelatedWork W1485699020 @default.
- W2013022634 hasRelatedWork W1501456700 @default.
- W2013022634 hasRelatedWork W1642205717 @default.
- W2013022634 hasRelatedWork W1981521205 @default.
- W2013022634 hasRelatedWork W2022214295 @default.
- W2013022634 hasRelatedWork W2029689644 @default.
- W2013022634 hasRelatedWork W2044239458 @default.
- W2013022634 hasRelatedWork W2087467041 @default.
- W2013022634 hasRelatedWork W2167404377 @default.
- W2013022634 hasRelatedWork W4253216837 @default.
- W2013022634 hasVolume "129" @default.
- W2013022634 isParatext "false" @default.
- W2013022634 isRetracted "false" @default.
- W2013022634 magId "2013022634" @default.
- W2013022634 workType "article" @default.