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- W2013036959 abstract "We report a simple and fast assay for DOVA which is determined spectrophotometrically as its benzoquinoxaline derivative formed by condensation with 2,3-diaminonaphthalene at pH 6–8. At such pH values any naturally-occurring 2-oxo-carboxylic acids, such as 2-oxoglutarate, are almost entirely ionized at the C-1 carboxyl group and are therefore unable to form benzoquinoxaline derivatives. Thus, in this method there is no need for the tedious separation by repetitive TLC of the DOVA benzoquinoxaline from those of the 2-oxocarboxylic acids as reported in earlier assays where the condensation with 2,3-diaminonaphthalene was performed in 1N-HC1. We also find this assay gives higher values than the older method because DOVA benzoquinoxaline is unstable in acid conditions. We report that DOVA has been detected by this method in Scenedesmus obliquas (mutant C-2A')." @default.
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- W2013036959 date "1980-01-01" @default.
- W2013036959 modified "2023-09-26" @default.
- W2013036959 title "A fast and convenient assay for 4,5-dioxovaleric acid in biological systems" @default.
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- W2013036959 doi "https://doi.org/10.1016/0020-711x(80)90154-8" @default.
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