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- W2013043737 abstract "The mustard trypsin inhibitor MTI2 was expressed as secretory protein in the yeast Pichia pastoris. In order to evaluate the influence of the C-terminal amino acids of the precursor form on the inhibitor activity, the C-terminal precursor and the mature protein were both expressed. A third His-tagged construct was also designed to compare alternative purification procedures. Proteins were efficiently expressed at levels of 40-160 mg/l in shake flasks. Equilibrium dissociation constants demonstrated that the mature protein was a stronger inhibitor of bovine beta-trypsin compared to the precursor and His-tagged forms (0.01 nM vs. 0.58 nM and 0.71 nM, respectively). The recombinant proteins were active inhibitors of Spodoptera exigua gut proteases." @default.
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- W2013043737 date "2000-02-22" @default.
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- W2013043737 title "Characterization of recombinant mustard trypsin inhibitor 2 (MTI2) expressed in <i>Pichia pastoris</i>" @default.
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- W2013043737 doi "https://doi.org/10.1016/s0014-5793(00)01207-2" @default.
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