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• W2013049639 abstract "The effect of chlorhexidine gluconate on the adherence of Candida albicans to human buccal epithelial cells (BEC) and drug-induced alterations in BEC membrane-lipid packing order were examined. Treatment of BEC with attached yeasts with 0.1 and 0.2% chlorhexidine resulted in significant yeast detachment after 90 and 60 min, respectively. Following pre-treatment of BEC with > 0.1% chlorhexidine, yeast adherence was inhibited by > 80%. In parallel experiments, the fluorescence anisotropy of BEC labeled with fluorescent membrane probes-diphenylhexatriene (DPH) and trimethylammonium DPH-was assessed following exposure to chlorhexidine. The fluorescence anisotropy decreased with increasing concentrations of chlorhexidine, which indicated that the drug decreased epithelial-cell membrane-lipid packing order. Chlorhexidine concentrations that altered epithelial-cell membrane-lipid packing order, particularly in superficial regions, were similar to those drug concentrations required for detachment of adherent yeasts. Similar results were obtained with a second antifungal, nystatin A. While the effects of chlorhexidine on the buccal-cell membrane-lipid packing order were not reversed by multiple washings, the opposite situation occurred with nystatin A. The results suggest that chlorhexidine-induced alterations ofBEC membrane-lipid order may be involved in the antifungal actions of the drug." @default.
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• W2013049639 date "1992-06-01" @default.
• W2013049639 modified "2023-09-27" @default.
• W2013049639 title "Chlorhexidine Effects on Membrane Lipid Domains of Human Buccal Epithelial Cells" @default.
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• W2013049639 doi "https://doi.org/10.1177/00220345920710060601" @default.
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