Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013054227> ?p ?o ?g. }
- W2013054227 endingPage "336" @default.
- W2013054227 startingPage "331" @default.
- W2013054227 abstract "Background:It has been proposed that cadmium (Cd) is an environmental “metalloestrogen” and that its action is mediated via the estrogen receptor (ER). Cd mimics the effects of estrogen in the rat uterus, and blood Cd concentrations positively correlate with ER levels in uteri of women with fibroids.Objectives:In the present study we explored whether Cd could stimulate proliferation of estrogen-responsive human uterine leiomyoma (ht-UtLM) cells and uterine smooth muscle cells (ht-UtSMCs) through classical interactions with ERα and ERβ, or by nongenomic mechanisms.Methods:We used estrogen response element (ERE) reporters, phosphorylated receptor tyrosine kinase arrays, Western blot analysis, estrogen binding, and cell proliferation assays to evaluate the effects of Cd on ht-UtLM cells and ht-UtSMCs.Results:Cd stimulated growth of both cell types at lower concentrations and inhibited growth at higher concentrations (≥ 50 μM). Cd did not significantly bind to ERα or ERβ, nor did it show transactivation in both cell types transiently transfected with ERE reporter genes. However, in both cells types, Cd (0.1 μM and 10 μM) activated p44/42 MAPK (ERK1/2), and a MAPK inhibitor (PD98059) abrogated Cd-induced cell proliferation. Cd in ht-UtLM cells, but not in ht-UtSMCs, activated the growth factor receptors EGFR, HGFR, and VEGF-R1 upstream of MAPK. Additional studies in ht-UtLM cells showed that AG1478, an EGFR inhibitor, abolished Cd-induced phosphorylation of EGFR and MAPK.Conclusions:Our results show that low concentrations of Cd stimulated cell proliferation in estrogen-responsive uterine cells by nongenomic activation of MAPK, but not through classical ER-mediated pathways.Citation:Gao X, Yu L, Moore AB, Kissling GE, Waalkes MP, Dixon D. 2015. Cadmium and proliferation in human uterine leiomyoma cells: evidence of a role for EGFR/MAPK pathways but not classical estrogen receptor pathways. Environ Health Perspect 123:331–336; http://dx.doi.org/10.1289/ehp.1408234" @default.
- W2013054227 created "2016-06-24" @default.
- W2013054227 creator A5009066152 @default.
- W2013054227 creator A5045523281 @default.
- W2013054227 creator A5070979789 @default.
- W2013054227 creator A5071207779 @default.
- W2013054227 creator A5079303450 @default.
- W2013054227 creator A5085886216 @default.
- W2013054227 date "2015-04-01" @default.
- W2013054227 modified "2023-10-16" @default.
- W2013054227 title "Cadmium and Proliferation in Human Uterine Leiomyoma Cells: Evidence of a Role for EGFR/MAPK Pathways but Not Classical Estrogen Receptor Pathways" @default.
- W2013054227 cites W123005099 @default.
- W2013054227 cites W1958574545 @default.
- W2013054227 cites W1965941649 @default.
- W2013054227 cites W1974562202 @default.
- W2013054227 cites W1989791107 @default.
- W2013054227 cites W1994062681 @default.
- W2013054227 cites W1994601047 @default.
- W2013054227 cites W2001338598 @default.
- W2013054227 cites W2004215518 @default.
- W2013054227 cites W2009687868 @default.
- W2013054227 cites W2013343669 @default.
- W2013054227 cites W2024795789 @default.
- W2013054227 cites W2025872894 @default.
- W2013054227 cites W2032080189 @default.
- W2013054227 cites W2032166158 @default.
- W2013054227 cites W2040024080 @default.
- W2013054227 cites W2043787658 @default.
- W2013054227 cites W2047620538 @default.
- W2013054227 cites W2054490367 @default.
- W2013054227 cites W2055094817 @default.
- W2013054227 cites W2060742283 @default.
- W2013054227 cites W2062289724 @default.
- W2013054227 cites W2064391759 @default.
- W2013054227 cites W2065337976 @default.
- W2013054227 cites W2072676812 @default.
- W2013054227 cites W2084637779 @default.
- W2013054227 cites W2085357207 @default.
- W2013054227 cites W2093488899 @default.
- W2013054227 cites W2094944215 @default.
- W2013054227 cites W2095454527 @default.
- W2013054227 cites W2098524712 @default.
- W2013054227 cites W2102884195 @default.
- W2013054227 cites W2111808706 @default.
- W2013054227 cites W2117642806 @default.
- W2013054227 cites W2118141024 @default.
- W2013054227 cites W2119745101 @default.
- W2013054227 cites W2121454015 @default.
- W2013054227 cites W2126625923 @default.
- W2013054227 cites W2130268528 @default.
- W2013054227 cites W2135278339 @default.
- W2013054227 cites W2157824687 @default.
- W2013054227 cites W2161821474 @default.
- W2013054227 cites W2163849415 @default.
- W2013054227 cites W2170353148 @default.
- W2013054227 cites W2171277104 @default.
- W2013054227 cites W2171661596 @default.
- W2013054227 cites W2332604002 @default.
- W2013054227 doi "https://doi.org/10.1289/ehp.1408234" @default.
- W2013054227 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4384203" @default.
- W2013054227 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25343777" @default.
- W2013054227 hasPublicationYear "2015" @default.
- W2013054227 type Work @default.
- W2013054227 sameAs 2013054227 @default.
- W2013054227 citedByCount "27" @default.
- W2013054227 countsByYear W20130542272015 @default.
- W2013054227 countsByYear W20130542272016 @default.
- W2013054227 countsByYear W20130542272017 @default.
- W2013054227 countsByYear W20130542272018 @default.
- W2013054227 countsByYear W20130542272019 @default.
- W2013054227 countsByYear W20130542272020 @default.
- W2013054227 countsByYear W20130542272021 @default.
- W2013054227 countsByYear W20130542272022 @default.
- W2013054227 crossrefType "journal-article" @default.
- W2013054227 hasAuthorship W2013054227A5009066152 @default.
- W2013054227 hasAuthorship W2013054227A5045523281 @default.
- W2013054227 hasAuthorship W2013054227A5070979789 @default.
- W2013054227 hasAuthorship W2013054227A5071207779 @default.
- W2013054227 hasAuthorship W2013054227A5079303450 @default.
- W2013054227 hasAuthorship W2013054227A5085886216 @default.
- W2013054227 hasBestOaLocation W20130542271 @default.
- W2013054227 hasConcept C104317684 @default.
- W2013054227 hasConcept C121608353 @default.
- W2013054227 hasConcept C126322002 @default.
- W2013054227 hasConcept C1292079 @default.
- W2013054227 hasConcept C134018914 @default.
- W2013054227 hasConcept C150194340 @default.
- W2013054227 hasConcept C185592680 @default.
- W2013054227 hasConcept C2777164284 @default.
- W2013054227 hasConcept C2779066055 @default.
- W2013054227 hasConcept C3017725331 @default.
- W2013054227 hasConcept C530470458 @default.
- W2013054227 hasConcept C55493867 @default.
- W2013054227 hasConcept C57074206 @default.
- W2013054227 hasConcept C62112901 @default.
- W2013054227 hasConcept C62478195 @default.
- W2013054227 hasConcept C71924100 @default.
- W2013054227 hasConcept C84606932 @default.