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- W2013071199 abstract "SYNOPSIS. Labeled fatty acids were produced by adding 10 mc acetate-1,2-C14 to cultures of Ochrosonas danica, with a recovery of 21.4% of the radioactivity as mixed methyl esters. Individual esters, or mixtures of isomers, were isolated in amounts between 2 and 200 mg by liquid-liquid chromatography followed by gas-liquid chromatography. Several esters, including Δ8,11,14-C20, Δ5,8,11,14,17-C20, Δ7,10,13,16-C22 and Δ4,7,10,13,18-C22 were obtained pure. Linoleate and arachidonate contained not more than 4% isomers; isomerism was more pronounced with C18:3 and C20:2. The effect of different culturing conditions on fatty acid yield, composition and incorporation of C14 was explored. The radioactive level of acids from the preparative run varied within a factor of 3, the average being about 1 μc/mg. All analytical and preparative procedures are described in detail and discussed." @default.
- W2013071199 created "2016-06-24" @default.
- W2013071199 creator A5073522366 @default.
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- W2013071199 date "1965-05-01" @default.
- W2013071199 modified "2023-10-14" @default.
- W2013071199 title "Preparation of Fatty Acids Labeled with C<sup>14</sup>from<i>Ochromonas danica</i>*" @default.
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- W2013071199 doi "https://doi.org/10.1111/j.1550-7408.1965.tb01834.x" @default.
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