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- W2013078475 abstract "Cholesterol efflux was studied in cultured Ob1771 adipose cells after preloading with LDL cholesterol. Exposure to particles containing apo AII (LpAI ) and particles containing apo AI and apo AII (LpAI : AII) isolated from native human plasma, and from HDL2 or HDL3, showed that only LpAI were able to promote cholesterol efflux, despite the fact that both kinds of particles were able to bind to receptor sites within the same range of concentrations (apparent Kd values between 10 and 25 μg/ml). During this long-term exposure, LpAI : AII demonstrated a concentration-dependent inhibition (10–60 μg/ml) of LpAI-mediated cholesterol efflux from adipose cells under conditions where LpAI : AII did not deliver cholesterol to the cells and where no net change in the distribution of apo AI between LpAI and LpAI : AII was observed. The antagonizing and modulating role of LpAI : AII in preventing cholesterol efflux mediated by LpAI appears not to be related to the lipid composition and cholesterol content of the particles but, rather, appears dependent upon the presence of apo AI in LpAI particles and apo AI in LpAI : AII particles. The actual concentrations of these particles in the interstitial fluid bathing peripheral cells might be critical for the in vivo occurrence of cholesterol efflux." @default.
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- W2013078475 date "1991-04-01" @default.
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- W2013078475 title "Differential role of apolipoprotein AI-containing particles in cholesterol efflux from adipose cells" @default.
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- W2013078475 doi "https://doi.org/10.1016/0021-9150(91)90016-v" @default.
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