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• W2013084721 abstract "A bstract : Traditional dogma has stated that space needs to be opened by cytoxic myeloablative therapy in order for marrow stem cells to engraft. Recent work in murine transplant models, however, indicates that engraftment is determined by the ratio of donor to host stem cells, i.e., stem cell competition. One hundred centigray whole body irradiation is stem cell toxic and nonmyelotoxic, thus allowing for higher donor chimerism in a murine syngeneic transplant setting. This nontoxic stem cell transplantation can be applied to allogeneic transplant with the addition of a tolerizing step; in this case presensitization with donor spleen cells and administration of CD40 ligand antibody to block costimulation. The stem cells that engraft in the nonmyeloablated are in G 0 , but are rapidly induced (by 12 hours) to enter the S phase after in vivo engraftment. Exposure of murine marrow to cytokines (IL‐3, IL‐6, IL‐11 and steel factor) expands progenitor clones, induces stem cells into cell cycle, and causes a fluctuating engraftment phenotype tied to phase of cell cycle. These data indicate that the concepts of stem cell competition and fluctuation of stem cell phenotype with cell cycle transit should underlie any new stem cell engraftment strategy." @default.
• W2013084721 created "2016-06-24" @default.
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• W2013084721 date "1999-04-01" @default.
• W2013084721 modified "2023-10-02" @default.
• W2013084721 title "Lymphohematopoietic Stem Cell Engraftmenta" @default.
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• W2013084721 doi "https://doi.org/10.1111/j.1749-6632.1999.tb08451.x" @default.
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