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- W2013087029 abstract "Abstract Glycosaminoglycans subjected to electrophoresis on cellulose acetate are stained by Alcian blue in the presence of different MgCl 2 concentrations. The procedure described solubilizes and elutes off the cellulose acetate strip glycosaminoglycans with critical electrolyte concentrations lower than the concentration of MgCl 2 , leaving stained on the strip glycosaminoglycans with higher critical electrolyte concentrations. This technique is particularly useful for sulfated glycosaminoglycans that are incompletely separated by electrophoresis and is applicable to the partial characterization of glycosaminoglycans down to a 0.1-μg range allowing practical quantitative studies of radioactively labeled glycosaminoglycans produced by low-density cell cultures. The staining technique is examined with respect to the specificity of the glycosaminoglycans eluted, and the nature of the interactions in the staining process is discussed. In addition, a simplified procedure is described for the characterization of glycosaminoglycans using testicular hyaluronidase (EC 3.2.1.35) and chondroitinase ABC (EC 4.2.2.4), which can be used in conjunction with the salt elution technique." @default.
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- W2013087029 date "1980-09-01" @default.
- W2013087029 modified "2023-09-23" @default.
- W2013087029 title "Characterization of glycosaminoglycan-alcian blue complexes by elution from cellulose acetate utilizing different MgCl2 concentrations" @default.
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- W2013087029 doi "https://doi.org/10.1016/0003-2697(80)90401-7" @default.
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