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- W2013090889 abstract "Particulate material from sonicated rat basophilic leukemia cells was isolated and examined for its 125I-IgE binding properties by a rapid filter assay. The binding was saturable and was stoichiometrically inhibited with unlabeled IgE. Binding parameters (at 37°C) were similar to those observed with intact cells though the forward rate constant was up to 2 × higher (2 × 105 M−1sec−1 while the backward rate constant was 2–3 × smaller (∼5 × 10−6 sec−1) yielding an estimated Ka of ∼4 × 1010M−1. The binding activity was irreversibly lost after brief exposure to temperatures above 50° and at pH's above 9 or below 5. The activity was largely resistant to proteolytic enzymes and neuraminidase, but not to phospholipase C. Non-ionic detergents led to apparent disruption of the particles." @default.
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- W2013090889 date "1976-05-01" @default.
- W2013090889 modified "2023-09-23" @default.
- W2013090889 title "Interaction of IgE with rat basophilic leukemia cells—v. Binding properties of cell free particles" @default.
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- W2013090889 doi "https://doi.org/10.1016/0019-2791(76)90377-3" @default.
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