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- W2013103692 abstract "Charcoal removed large portions of the inhibitory activity from the egg extracts. The substances eluted from the charcoal and lyophilized produced very little retardation of cleavage by themselves in comparison with that expected from the original extract, yet when reduced glutathione was added along with the eluted substances, a delay occurred (Table V). Discussion-Several of the characteristics of the activity of the retarding factor in sea urchin eggs suggest that the retardation of cleavage induced by egg extracts is associated with the sulfhydryl groups. Agents which would be expected to maintain the sulfhydryl groups of the extract in reduced form (reduced glutathione, cysteine) increased the delay in cleavage. Agents which would oxidize the sulfhydryl groups (oxidized glutathione, iodosobenzoic acid, aeration) decreased the delay. The fact that the extract is more inhibitory in less alkaline media [5] is also in accord with the suggestion that sulfhydryl groups are involved, since sulfhydryl groups are more stable in less alkaline solutions [4]." @default.
- W2013103692 created "2016-06-24" @default.
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- W2013103692 date "1960-10-01" @default.
- W2013103692 modified "2023-09-26" @default.
- W2013103692 title "Incorporation of labelled amino acids into the proteins of the mitochondria isolated from the unfertilized eggs and developmental stages of Paracentrotus lividus" @default.
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- W2013103692 doi "https://doi.org/10.1016/0014-4827(60)90368-2" @default.
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