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- W2013122679 abstract "We demonstrate for the first time that functional electrogenic Na(+)-dependent amino acid transport is preserved for extended periods when purified brush border membranes prepared in hypotonic media are lyophilized and then rehydrated in buffer containing mannitol, NaSCN, and/or KSCN/valinomycin. Reconstituted lyophilized apical membranes from small intestine formed morphologically, physiologically, and thermodynamically normal vesicles which transported L-alanine via system B into an osmotically active space energized by secondary active transport, as measured under equilibrium and nonequilibrium conditions. The lyophilized membranes are readily prepared and stored, thereby providing a means to pool large quantities of formed vesicles that are useful in examining cloned and reconstituted native amino acid transporter polypeptides." @default.
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- W2013122679 modified "2023-10-06" @default.
- W2013122679 title "Sodium-Dependent Amino Acid Transport Is Preserved in Lyophilized Reconstituted Apical Membranes from Intestinal Epithelium" @default.
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- W2013122679 doi "https://doi.org/10.1006/abio.1998.2862" @default.
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