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- W2013129488 abstract "A method has been developed that allows the isolation of genomic clones from a cosmid library by homologous recombination in vivo. This method was used to isolate a human genomic interleukin 2 (IL2) gene. The genomic cosmid library was packaged in vivo into lambda phage particles. A recombination-proficient host strain carrying IL2 cDNA sequences in a non-homologous plasmid vector was infected by the packaged cosmid library. After in vivo packaging and reinfection, recombinants carrying the antibiotic resistance genes of both vectors were selected. From a recombinant cosmid clone the chromosomal IL2 gene was restored. After DNA mediated gene transfer into mouse Ltk- cells human IL2 was expressed constitutively." @default.
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- W2013129488 date "1985-01-01" @default.
- W2013129488 modified "2023-10-09" @default.
- W2013129488 title "Isolation of a functional human interleukin 2 gene from a cosmid library by recombination in vivo" @default.
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- W2013129488 doi "https://doi.org/10.1016/0378-1119(85)90104-0" @default.
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