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- W2013135488 abstract "Baculoviruses have a unique bi-phasic life cycle and powerful promoters, which greatly facilitates their use for recombinant protein expression in insect cells. We have developed an expression system that utilizes homologous recombination in insect cells between a transfer plasmid containing a gene to be expressed and a replication-deficient virus (bacmid). Only recombinant virus can replicate facilitating the rapid production of multiple recombinant viruses using robotic liquid handlers. The bacmid has also been genetically optimized for improved protein expression and stability. We describe the application of this system for high level production of recombinant proteins." @default.
- W2013135488 created "2016-06-24" @default.
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- W2013135488 date "2011-09-01" @default.
- W2013135488 modified "2023-10-17" @default.
- W2013135488 title "Optimizing the baculovirus expression vector system" @default.
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- W2013135488 doi "https://doi.org/10.1016/j.ymeth.2011.06.011" @default.
- W2013135488 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21945427" @default.
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