FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013171335> ?p ?o ?g. }

• W2013171335 endingPage "349" @default.
• W2013171335 startingPage "343" @default.
• W2013171335 abstract "Previous studies together with ours showed that heat shock protein gp96 as an adjuvant induces antigen specific T cell responses against cancer and infectious diseases. However, at present there is no efficient method to obtain high amount of full-length gp96 by in vitro expression. Here, we used the yeast Hansenula polymorpha as an efficient host for gp96 recombinant protein production. The transformant clones with highly expressed recombinant proteins were screened and selected by measuring the halo size which indicates enzymatic hydrolysis of starch in the medium. High-level production of gp96 (around 150 mg/mL) was achieved by using high-cell density fed-batch cultivations. We showed that peptide binding of the recombinant protein has similar specificity and intrinsic binding parameters as that of the native gp96. We next examined the self-assembly properties and high-order structures of the recombinant protein. Moreover, the H. polymorpha expressed recombinant gp96 can effectively induce HBV-specific CTL response in immunized mice while Escherichia coli-expressed gp96 cannot. Our results therefore may provide bases for structure and functional studies of gp96 and thereby potentially expedite the development of gp96-based vaccines for immunotherapy of cancer or infectious diseases." @default.
• W2013171335 created "2016-06-24" @default.
• W2013171335 creator A5005050685 @default.
• W2013171335 creator A5005678038 @default.
• W2013171335 creator A5019466654 @default.
• W2013171335 creator A5023512298 @default.
• W2013171335 creator A5027670496 @default.
• W2013171335 creator A5028179790 @default.
• W2013171335 creator A5053708842 @default.
• W2013171335 creator A5059854137 @default.
• W2013171335 creator A5077589994 @default.
• W2013171335 creator A5083531522 @default.
• W2013171335 creator A5089883179 @default.
• W2013171335 date "2011-02-01" @default.
• W2013171335 modified "2023-09-26" @default.
• W2013171335 title "Hansenula polymorpha expressed heat shock protein gp96 exerts potent T cell activation activity as an adjuvant" @default.
• W2013171335 cites W1526294038 @default.
• W2013171335 cites W1540090690 @default.
• W2013171335 cites W1546757669 @default.
• W2013171335 cites W1658799699 @default.
• W2013171335 cites W1968020948 @default.
• W2013171335 cites W1973426512 @default.
• W2013171335 cites W1993204706 @default.
• W2013171335 cites W2004374675 @default.
• W2013171335 cites W2004635577 @default.
• W2013171335 cites W2005266650 @default.
• W2013171335 cites W2007743840 @default.
• W2013171335 cites W2015020062 @default.
• W2013171335 cites W2019713801 @default.
• W2013171335 cites W2021632324 @default.
• W2013171335 cites W2023089746 @default.
• W2013171335 cites W2034498656 @default.
• W2013171335 cites W2042204926 @default.
• W2013171335 cites W2048718918 @default.
• W2013171335 cites W2054626405 @default.
• W2013171335 cites W2059361896 @default.
• W2013171335 cites W2072115152 @default.
• W2013171335 cites W2085758550 @default.
• W2013171335 cites W2102403552 @default.
• W2013171335 cites W2109648512 @default.
• W2013171335 cites W2119277511 @default.
• W2013171335 cites W2124784272 @default.
• W2013171335 cites W2138886006 @default.
• W2013171335 cites W2140267818 @default.
• W2013171335 cites W2152702111 @default.
• W2013171335 cites W2160797991 @default.
• W2013171335 cites W2163615393 @default.
• W2013171335 cites W2168382431 @default.
• W2013171335 cites W2177209358 @default.
• W2013171335 doi "https://doi.org/10.1016/j.jbiotec.2010.12.006" @default.
• W2013171335 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/21167226" @default.
• W2013171335 hasPublicationYear "2011" @default.
• W2013171335 type Work @default.
• W2013171335 sameAs 2013171335 @default.
• W2013171335 citedByCount "17" @default.
• W2013171335 countsByYear W20131713352012 @default.
• W2013171335 countsByYear W20131713352013 @default.
• W2013171335 countsByYear W20131713352014 @default.
• W2013171335 countsByYear W20131713352016 @default.
• W2013171335 countsByYear W20131713352018 @default.
• W2013171335 countsByYear W20131713352019 @default.
• W2013171335 countsByYear W20131713352021 @default.
• W2013171335 crossrefType "journal-article" @default.
• W2013171335 hasAuthorship W2013171335A5005050685 @default.
• W2013171335 hasAuthorship W2013171335A5005678038 @default.
• W2013171335 hasAuthorship W2013171335A5019466654 @default.
• W2013171335 hasAuthorship W2013171335A5023512298 @default.
• W2013171335 hasAuthorship W2013171335A5027670496 @default.
• W2013171335 hasAuthorship W2013171335A5028179790 @default.
• W2013171335 hasAuthorship W2013171335A5053708842 @default.
• W2013171335 hasAuthorship W2013171335A5059854137 @default.
• W2013171335 hasAuthorship W2013171335A5077589994 @default.
• W2013171335 hasAuthorship W2013171335A5083531522 @default.
• W2013171335 hasAuthorship W2013171335A5089883179 @default.
• W2013171335 hasConcept C104317684 @default.
• W2013171335 hasConcept C147483822 @default.
• W2013171335 hasConcept C153911025 @default.
• W2013171335 hasConcept C203014093 @default.
• W2013171335 hasConcept C205260736 @default.
• W2013171335 hasConcept C2777863537 @default.
• W2013171335 hasConcept C2779222958 @default.
• W2013171335 hasConcept C40767141 @default.
• W2013171335 hasConcept C547475151 @default.
• W2013171335 hasConcept C55493867 @default.
• W2013171335 hasConcept C86803240 @default.
• W2013171335 hasConceptScore W2013171335C104317684 @default.
• W2013171335 hasConceptScore W2013171335C147483822 @default.
• W2013171335 hasConceptScore W2013171335C153911025 @default.
• W2013171335 hasConceptScore W2013171335C203014093 @default.
• W2013171335 hasConceptScore W2013171335C205260736 @default.
• W2013171335 hasConceptScore W2013171335C2777863537 @default.
• W2013171335 hasConceptScore W2013171335C2779222958 @default.
• W2013171335 hasConceptScore W2013171335C40767141 @default.
• W2013171335 hasConceptScore W2013171335C547475151 @default.
• W2013171335 hasConceptScore W2013171335C55493867 @default.
• W2013171335 hasConceptScore W2013171335C86803240 @default.
• W2013171335 hasIssue "4" @default.
• W2013171335 hasLocation W20131713351 @default.

• next