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• W2013173675 abstract "The classical model of secretory vesicle recycling after exocytosis involves the retrieval of membrane (the omega figure) at a different site. An alternative model involves secretory vesicles transiently fusing with the plasma membrane (the ‘kiss and run’ mechanism) [1Neher E Cell physiology. Secretion without full fusion.Nature. 1993; 363: 497-498Crossref PubMed Scopus (95) Google Scholar, 2Almers W Tse FW Transmitter release from synapses: does a preassembled fusion pore initiate exocytosis?.Neuron. 1990; 4: 813-818Abstract Full Text PDF PubMed Scopus (137) Google Scholar]. No continuous observation of the fate of a single secretory vesicle after exocytosis has been made to date. To study the dynamics of fusion immediately following exocytosis of insulin-containing vesicles, enhanced green fluorescent protein (EGFP) fused to the vesicle membrane protein phogrin [[3]Pouli AE Emmanouilidou E Zhao C Wasmeier C Hutton JC Rutter GA Secretory-granule dynamics visualized in vivo with a phogrin-green fluorescent protein chimaera.Biochem J. 1998; 333: 193-199Crossref PubMed Scopus (125) Google Scholar] was delivered to the secretory vesicle membrane of INS-1 β-cells using an adenoviral vector. The behaviour of the vesicle membrane during single exocytotic events was then examined using evanescent wave microscopy [4Steyer JA Horstmann H Almers W Transport, docking and exocytosis of single secretory granules in live chromaffin cells.Nature. 1997; 388: 474-478Crossref PubMed Scopus (360) Google Scholar, 5Toomre D Steyer JA Keller P Almers W Simons K Fusion of constitutive membrane traffic with the cell surface observed by evanescent wave microscopy.J Cell Biol. 2000; 149: 33-40Crossref PubMed Scopus (136) Google Scholar, 6Schmoranzer J Goulian M Axelrod D Simon SM Imaging constitutive exocytosis with total internal reflection fluorescence microscopy.J Cell Biol. 2000; 149: 23-32Crossref PubMed Scopus (156) Google Scholar]. In unstimulated cells, secretory vesicles showed only slow Brownian movement. After a depolarizing pulse, most vesicles showed a small decrease in phogrin–EGFP fluorescence, and some moved laterally over the plasma membrane for ∼1μm. In contrast, secretory vesicles loaded with acridine orange all showed a transient (33–100ms) increase in fluorescence intensity followed by rapid disappearance. Simultaneous observations of phogrin–EGFP and acridine orange indicated that the decrease in EGFP fluorescence occurred at the time of the acridine orange release, and that the lateral movement of EGFP-expressing vesicles occurred after this. Post-exocytotic retrieval of the vesicle membrane in INS-1 cells is thus slow, and can involve the movement of empty vesicles under the plasma membrane (‘kiss and glide’)." @default.
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• W2013173675 title "Simultaneous evanescent wave imaging of insulin vesicle membrane and cargo during a single exocytotic event" @default.
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• W2013173675 doi "https://doi.org/10.1016/s0960-9822(00)00756-9" @default.
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