Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013181947> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2013181947 endingPage "130" @default.
- W2013181947 startingPage "121" @default.
- W2013181947 abstract "Isolation of microglia from CNS tissue provides a powerful tool to study basic microglia biology and examine the effects of in vivo treatments on microglia immunophenotype and function. Previous microglia isolation methodologies utilized whole brain. However, microglia immunophenotype varies across CNS anatomical loci, thus isolation of microglia from whole brain may obscure regional brain variations in microglia immunophenotype and function. In addition, it is unknown to what extent microglia isolation procedures alter the in situ immunophenotype and function of microglia. The present report details a procedure for the rapid isolation of microglia from discrete CNS anatomical loci and addresses the issue of whether the in situ microglia immunophenotype is significantly altered by the isolation procedure. The present microglia isolation method yielded highly enriched hippocampal microglia, which were devoid of other CNS macrophage subtypes and exhibited attributes reflecting a quiescent phenotype characteristic of microglia observed in situ under non-pathological conditions. Further, isolated microglia exhibited functional responsiveness to immunogenic stimuli ex vivo. The immunophenotypic and functional attributes of isolated microglia suggest that the isolation procedure preserves the in vivo phenotype of microglia, thus providing an experimental method with minimal procedural confounds for examining in vivo treatments on microglia ex vivo." @default.
- W2013181947 created "2016-06-24" @default.
- W2013181947 creator A5011957342 @default.
- W2013181947 creator A5025282582 @default.
- W2013181947 creator A5046557028 @default.
- W2013181947 creator A5060565859 @default.
- W2013181947 date "2006-03-01" @default.
- W2013181947 modified "2023-10-14" @default.
- W2013181947 title "Rapid isolation of highly enriched and quiescent microglia from adult rat hippocampus: Immunophenotypic and functional characteristics" @default.
- W2013181947 cites W1986160938 @default.
- W2013181947 cites W1991805788 @default.
- W2013181947 cites W2000976612 @default.
- W2013181947 cites W2009199474 @default.
- W2013181947 cites W2009230499 @default.
- W2013181947 cites W2018766926 @default.
- W2013181947 cites W2041112741 @default.
- W2013181947 cites W2044315549 @default.
- W2013181947 cites W2060448382 @default.
- W2013181947 cites W2100358447 @default.
- W2013181947 cites W2107277218 @default.
- W2013181947 cites W2109759840 @default.
- W2013181947 cites W2139023977 @default.
- W2013181947 cites W2158714788 @default.
- W2013181947 cites W2341294987 @default.
- W2013181947 cites W4210981518 @default.
- W2013181947 cites W4244847225 @default.
- W2013181947 cites W4294216491 @default.
- W2013181947 doi "https://doi.org/10.1016/j.jneumeth.2005.06.026" @default.
- W2013181947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/16125247" @default.
- W2013181947 hasPublicationYear "2006" @default.
- W2013181947 type Work @default.
- W2013181947 sameAs 2013181947 @default.
- W2013181947 citedByCount "150" @default.
- W2013181947 countsByYear W20131819472012 @default.
- W2013181947 countsByYear W20131819472013 @default.
- W2013181947 countsByYear W20131819472014 @default.
- W2013181947 countsByYear W20131819472015 @default.
- W2013181947 countsByYear W20131819472016 @default.
- W2013181947 countsByYear W20131819472017 @default.
- W2013181947 countsByYear W20131819472018 @default.
- W2013181947 countsByYear W20131819472019 @default.
- W2013181947 countsByYear W20131819472020 @default.
- W2013181947 countsByYear W20131819472021 @default.
- W2013181947 countsByYear W20131819472022 @default.
- W2013181947 countsByYear W20131819472023 @default.
- W2013181947 crossrefType "journal-article" @default.
- W2013181947 hasAuthorship W2013181947A5011957342 @default.
- W2013181947 hasAuthorship W2013181947A5025282582 @default.
- W2013181947 hasAuthorship W2013181947A5046557028 @default.
- W2013181947 hasAuthorship W2013181947A5060565859 @default.
- W2013181947 hasConcept C142724271 @default.
- W2013181947 hasConcept C147483822 @default.
- W2013181947 hasConcept C148762608 @default.
- W2013181947 hasConcept C169760540 @default.
- W2013181947 hasConcept C196166836 @default.
- W2013181947 hasConcept C203014093 @default.
- W2013181947 hasConcept C207001950 @default.
- W2013181947 hasConcept C26291073 @default.
- W2013181947 hasConcept C2776914184 @default.
- W2013181947 hasConcept C2779830541 @default.
- W2013181947 hasConcept C54355233 @default.
- W2013181947 hasConcept C71924100 @default.
- W2013181947 hasConcept C86803240 @default.
- W2013181947 hasConcept C87753298 @default.
- W2013181947 hasConceptScore W2013181947C142724271 @default.
- W2013181947 hasConceptScore W2013181947C147483822 @default.
- W2013181947 hasConceptScore W2013181947C148762608 @default.
- W2013181947 hasConceptScore W2013181947C169760540 @default.
- W2013181947 hasConceptScore W2013181947C196166836 @default.
- W2013181947 hasConceptScore W2013181947C203014093 @default.
- W2013181947 hasConceptScore W2013181947C207001950 @default.
- W2013181947 hasConceptScore W2013181947C26291073 @default.
- W2013181947 hasConceptScore W2013181947C2776914184 @default.
- W2013181947 hasConceptScore W2013181947C2779830541 @default.
- W2013181947 hasConceptScore W2013181947C54355233 @default.
- W2013181947 hasConceptScore W2013181947C71924100 @default.
- W2013181947 hasConceptScore W2013181947C86803240 @default.
- W2013181947 hasConceptScore W2013181947C87753298 @default.
- W2013181947 hasIssue "2" @default.
- W2013181947 hasLocation W20131819471 @default.
- W2013181947 hasLocation W20131819472 @default.
- W2013181947 hasOpenAccess W2013181947 @default.
- W2013181947 hasPrimaryLocation W20131819471 @default.
- W2013181947 hasRelatedWork W2001635982 @default.
- W2013181947 hasRelatedWork W2058986539 @default.
- W2013181947 hasRelatedWork W2092427927 @default.
- W2013181947 hasRelatedWork W2137183077 @default.
- W2013181947 hasRelatedWork W2893560416 @default.
- W2013181947 hasRelatedWork W2913737174 @default.
- W2013181947 hasRelatedWork W2931439260 @default.
- W2013181947 hasRelatedWork W3107895302 @default.
- W2013181947 hasRelatedWork W3196574558 @default.
- W2013181947 hasRelatedWork W4319304529 @default.
- W2013181947 hasVolume "151" @default.
- W2013181947 isParatext "false" @default.
- W2013181947 isRetracted "false" @default.
- W2013181947 magId "2013181947" @default.
- W2013181947 workType "article" @default.