Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013184645> ?p ?o ?g. }
Showing items 1 to 81 of
81
with 100 items per page.
- W2013184645 endingPage "193" @default.
- W2013184645 startingPage "187" @default.
- W2013184645 abstract "The female guinea pig kidney cytosol and microsomes contain a trace or no detectable NADP+ or NAD+-linked dehydrogenase activity for several hydroxy-C19-steroids. The administration of testosterone induced a gradual increase in the 17β-C19-steroid dehydrogenase activity of the cytosol, but not of the microsomes, to the level in the male after 50 days. The induced enzyme was purified by (NH4)2SO4 precipitation, Sephadex G-75 nitration and DEAE-Sephadex A-50 and CM-Sephadex C-50 chromatography to give a protein which gave a molecular weight of 32,000 and a single protein and enzyme staining band on gel electrophoresis. The pure enzyme dissociated into three gel electrophoretic bands on removal of 2-mercaptoethanol from the solution and was restored by replacement of the mercaptoethanol. The enzyme in the cytosol, however, dissociated on storage at 4°C for 48 h or longer in the presence or absence of 2-mercaptoethanol. The KM value, steroid specificity, gel electrophoretic pattern, isoelectric value, pH optimum and NADP+ and NAD+ requirement were practically identical with those of the purified major 17β-hydroxy-C19-steroid dehydrogenase of the male guinea pig kidney. The several hydroxy-C19-steroid dehydrogenase activities of the liver were not influenced by testosterone administration. The highest enzyme activity was exhibited with 5β-dihydrotestosterone as substrate." @default.
- W2013184645 created "2016-06-24" @default.
- W2013184645 creator A5001002431 @default.
- W2013184645 creator A5027107107 @default.
- W2013184645 date "1979-02-01" @default.
- W2013184645 modified "2023-09-27" @default.
- W2013184645 title "The induction, purification and characterization of 17β-hydroxy-C19-steroid dehydrogenase of the female guinea pig kidney" @default.
- W2013184645 cites W1524996052 @default.
- W2013184645 cites W2000122018 @default.
- W2013184645 cites W2039657724 @default.
- W2013184645 cites W2051327961 @default.
- W2013184645 cites W2077449350 @default.
- W2013184645 cites W2082522861 @default.
- W2013184645 cites W2328475260 @default.
- W2013184645 cites W2407993996 @default.
- W2013184645 doi "https://doi.org/10.1016/0022-4731(79)90234-6" @default.
- W2013184645 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/513731" @default.
- W2013184645 hasPublicationYear "1979" @default.
- W2013184645 type Work @default.
- W2013184645 sameAs 2013184645 @default.
- W2013184645 citedByCount "12" @default.
- W2013184645 countsByYear W20131846452020 @default.
- W2013184645 crossrefType "journal-article" @default.
- W2013184645 hasAuthorship W2013184645A5001002431 @default.
- W2013184645 hasAuthorship W2013184645A5027107107 @default.
- W2013184645 hasConcept C134018914 @default.
- W2013184645 hasConcept C153911025 @default.
- W2013184645 hasConcept C163994747 @default.
- W2013184645 hasConcept C181199279 @default.
- W2013184645 hasConcept C185592680 @default.
- W2013184645 hasConcept C2776317432 @default.
- W2013184645 hasConcept C2778815084 @default.
- W2013184645 hasConcept C2780902042 @default.
- W2013184645 hasConcept C28212737 @default.
- W2013184645 hasConcept C43617362 @default.
- W2013184645 hasConcept C50476208 @default.
- W2013184645 hasConcept C55493867 @default.
- W2013184645 hasConcept C71315377 @default.
- W2013184645 hasConcept C75520062 @default.
- W2013184645 hasConcept C86803240 @default.
- W2013184645 hasConcept C87644729 @default.
- W2013184645 hasConcept C98539663 @default.
- W2013184645 hasConceptScore W2013184645C134018914 @default.
- W2013184645 hasConceptScore W2013184645C153911025 @default.
- W2013184645 hasConceptScore W2013184645C163994747 @default.
- W2013184645 hasConceptScore W2013184645C181199279 @default.
- W2013184645 hasConceptScore W2013184645C185592680 @default.
- W2013184645 hasConceptScore W2013184645C2776317432 @default.
- W2013184645 hasConceptScore W2013184645C2778815084 @default.
- W2013184645 hasConceptScore W2013184645C2780902042 @default.
- W2013184645 hasConceptScore W2013184645C28212737 @default.
- W2013184645 hasConceptScore W2013184645C43617362 @default.
- W2013184645 hasConceptScore W2013184645C50476208 @default.
- W2013184645 hasConceptScore W2013184645C55493867 @default.
- W2013184645 hasConceptScore W2013184645C71315377 @default.
- W2013184645 hasConceptScore W2013184645C75520062 @default.
- W2013184645 hasConceptScore W2013184645C86803240 @default.
- W2013184645 hasConceptScore W2013184645C87644729 @default.
- W2013184645 hasConceptScore W2013184645C98539663 @default.
- W2013184645 hasIssue "2" @default.
- W2013184645 hasLocation W20131846451 @default.
- W2013184645 hasLocation W20131846452 @default.
- W2013184645 hasOpenAccess W2013184645 @default.
- W2013184645 hasPrimaryLocation W20131846451 @default.
- W2013184645 hasRelatedWork W1674335667 @default.
- W2013184645 hasRelatedWork W1974254036 @default.
- W2013184645 hasRelatedWork W1977200340 @default.
- W2013184645 hasRelatedWork W2010372900 @default.
- W2013184645 hasRelatedWork W2024817429 @default.
- W2013184645 hasRelatedWork W2030492692 @default.
- W2013184645 hasRelatedWork W2042153386 @default.
- W2013184645 hasRelatedWork W2063845813 @default.
- W2013184645 hasRelatedWork W2083360052 @default.
- W2013184645 hasRelatedWork W2397786582 @default.
- W2013184645 hasVolume "10" @default.
- W2013184645 isParatext "false" @default.
- W2013184645 isRetracted "false" @default.
- W2013184645 magId "2013184645" @default.
- W2013184645 workType "article" @default.