FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013203608> ?p ?o ?g. }

• W2013203608 endingPage "1296" @default.
• W2013203608 startingPage "1287" @default.
• W2013203608 abstract "The beta-D-endoglycosidase heparanase has been proposed as an important contributor to loss of glomerular charge in proteinuria. Expression of heparanase was, therefore, determined in acute puromycin aminonucleoside (PAN) nephrosis.A rabbit polyclonal antibody was produced against a 17-amino acid peptide derived from the predicted amino acid sequence of heparanase. The antibody was validated by Western blot. Immunohistochemical staining and Western blotting were used to localize heparanase protein in normal kidneys and kidneys from rats with PAN nephrosis. Northern blot analysis was used to determine mRNA expression.Immunohistochemical staining showed that heparanase protein was localized to tubular cells in the distal convoluted tubules, thick ascending limb of the loop of Henle, and transitional cell epithelium in normal kidney. Minimal expression was noted in normal glomeruli. Western blot analysis of protein from isolated normal glomeruli showed minimal expression of the 65 kD proheparanase protein. A marked increase in the staining for heparanase was found at day 5 of the PAN nephrosis model, at approximately the time of onset of proteinuria, and at day 14. Expression was predominantly seen in podocytes. At day 5, only the 65 kD proheparanase species was identified, but at day 14, mature 58 kD heparanase also was present. Northern blot analysis of sieved glomeruli at day 14 confirmed an increase in heparanase mRNA. The human podocyte cell line 56/10A1 also produced both proheparanase and mature heparanase, suggesting that podocytes can activate heparanase without other cell types.The previously mentioned data confirm that the novel beta-D-endoglycosidase heparanase is up-regulated and activated in glomeruli from rats with proteinuria. Heparanase may be involved, therefore, in the loss of glomerular charge seen in proteinuria. Moreover, the presence of heparanase in normal tubules suggests that it may also be involved in cell migration or turnover." @default.
• W2013203608 created "2016-06-24" @default.
• W2013203608 creator A5004347431 @default.
• W2013203608 creator A5024058809 @default.
• W2013203608 creator A5058161492 @default.
• W2013203608 creator A5084877588 @default.
• W2013203608 date "2001-10-01" @default.
• W2013203608 modified "2023-10-14" @default.
• W2013203608 title "Increased expression of heparanase in puromycin aminonucleoside nephrosis" @default.
• W2013203608 cites W1491748226 @default.
• W2013203608 cites W1493336035 @default.
• W2013203608 cites W1526312778 @default.
• W2013203608 cites W1869562643 @default.
• W2013203608 cites W1968528145 @default.
• W2013203608 cites W1975370922 @default.
• W2013203608 cites W1983244507 @default.
• W2013203608 cites W1993165881 @default.
• W2013203608 cites W2003929885 @default.
• W2013203608 cites W2004329227 @default.
• W2013203608 cites W2008406499 @default.
• W2013203608 cites W2017554243 @default.
• W2013203608 cites W2019565365 @default.
• W2013203608 cites W2021009141 @default.
• W2013203608 cites W2021904282 @default.
• W2013203608 cites W2027789202 @default.
• W2013203608 cites W2057107220 @default.
• W2013203608 cites W2079579615 @default.
• W2013203608 cites W2090955625 @default.
• W2013203608 cites W2093454357 @default.
• W2013203608 cites W2105301755 @default.
• W2013203608 cites W2109668012 @default.
• W2013203608 cites W2135845349 @default.
• W2013203608 cites W2136530946 @default.
• W2013203608 cites W2145367943 @default.
• W2013203608 cites W2218345254 @default.
• W2013203608 cites W2275451044 @default.
• W2013203608 doi "https://doi.org/10.1046/j.1523-1755.2001.00934.x" @default.
• W2013203608 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11576343" @default.
• W2013203608 hasPublicationYear "2001" @default.
• W2013203608 type Work @default.
• W2013203608 sameAs 2013203608 @default.
• W2013203608 citedByCount "64" @default.
• W2013203608 countsByYear W20132036082012 @default.
• W2013203608 countsByYear W20132036082013 @default.
• W2013203608 countsByYear W20132036082015 @default.
• W2013203608 countsByYear W20132036082016 @default.
• W2013203608 countsByYear W20132036082017 @default.
• W2013203608 countsByYear W20132036082018 @default.
• W2013203608 countsByYear W20132036082019 @default.
• W2013203608 countsByYear W20132036082020 @default.
• W2013203608 countsByYear W20132036082021 @default.
• W2013203608 countsByYear W20132036082022 @default.
• W2013203608 countsByYear W20132036082023 @default.
• W2013203608 crossrefType "journal-article" @default.
• W2013203608 hasAuthorship W2013203608A5004347431 @default.
• W2013203608 hasAuthorship W2013203608A5024058809 @default.
• W2013203608 hasAuthorship W2013203608A5058161492 @default.
• W2013203608 hasAuthorship W2013203608A5084877588 @default.
• W2013203608 hasBestOaLocation W20132036081 @default.
• W2013203608 hasConcept C104317684 @default.
• W2013203608 hasConcept C105580179 @default.
• W2013203608 hasConcept C126322002 @default.
• W2013203608 hasConcept C134018914 @default.
• W2013203608 hasConcept C1491633281 @default.
• W2013203608 hasConcept C153911025 @default.
• W2013203608 hasConcept C177707886 @default.
• W2013203608 hasConcept C203014093 @default.
• W2013203608 hasConcept C204232928 @default.
• W2013203608 hasConcept C2776415932 @default.
• W2013203608 hasConcept C2777146197 @default.
• W2013203608 hasConcept C2777478136 @default.
• W2013203608 hasConcept C2778041096 @default.
• W2013203608 hasConcept C2779200571 @default.
• W2013203608 hasConcept C2779561371 @default.
• W2013203608 hasConcept C2780091579 @default.
• W2013203608 hasConcept C2781175652 @default.
• W2013203608 hasConcept C3675279 @default.
• W2013203608 hasConcept C55493867 @default.
• W2013203608 hasConcept C71924100 @default.
• W2013203608 hasConcept C85265743 @default.
• W2013203608 hasConcept C86803240 @default.
• W2013203608 hasConceptScore W2013203608C104317684 @default.
• W2013203608 hasConceptScore W2013203608C105580179 @default.
• W2013203608 hasConceptScore W2013203608C126322002 @default.
• W2013203608 hasConceptScore W2013203608C134018914 @default.
• W2013203608 hasConceptScore W2013203608C1491633281 @default.
• W2013203608 hasConceptScore W2013203608C153911025 @default.
• W2013203608 hasConceptScore W2013203608C177707886 @default.
• W2013203608 hasConceptScore W2013203608C203014093 @default.
• W2013203608 hasConceptScore W2013203608C204232928 @default.
• W2013203608 hasConceptScore W2013203608C2776415932 @default.
• W2013203608 hasConceptScore W2013203608C2777146197 @default.
• W2013203608 hasConceptScore W2013203608C2777478136 @default.
• W2013203608 hasConceptScore W2013203608C2778041096 @default.
• W2013203608 hasConceptScore W2013203608C2779200571 @default.
• W2013203608 hasConceptScore W2013203608C2779561371 @default.
• W2013203608 hasConceptScore W2013203608C2780091579 @default.
• W2013203608 hasConceptScore W2013203608C2781175652 @default.

• next