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- W2013209285 abstract "We have previously shown that lamellar body-like particles, the form in which pulmonary surfactant is secreted, spontaneously disintegrate when they contact an air-liquid interface, eventually creating an interfacial film. Here, we combined these studies with a new technique enabling the simultaneous and non-invasive measurement of surface tension (γ). This method is a refinement of the pendant-drop principle. A sapphire cone with a 300-μm aperture keeps the experimental fluid by virtue of surface coherence in a fixed and nearly planar position above the objective of an inverted microscope. The radius of curvature of the fluid meniscus is related to γ and determines the pattern of light back-reflection upon epi-illumination. This method, which we name “inverted interface”, has several novel aspects, in particular its microscopic dimensions. When using lamellar body-like particles freshly released by alveolar type II cells, we found that their conversion at the interface resulted in γ-reduction close to 30 mN/m. After a fast initial decay, γ-decrease proceeded slowly and in proportion to single particle conversions. These conversions ceased with time whereas γ decreased further, probably due to reorganization of the already deposited material. The present investigation indicates that surface film formation by adsorption of large surfactant aggregates is an important mechanism by which γ is reduced in the lung." @default.
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- W2013209285 date "2005-08-01" @default.
- W2013209285 modified "2023-10-15" @default.
- W2013209285 title "Optical Measurement of Surface Tension in a Miniaturized Air-Liquid Interface and its Application in Lung Physiology" @default.
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- W2013209285 doi "https://doi.org/10.1529/biophysj.104.053132" @default.
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