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- W2013235514 abstract "Oxytocin and arginine-vasopressin (AVP) are secreted into the blood in low concentrations. To analyze these peptides, we investigated two common extraction procedures, acetone-ether precipitation and C(18)-SepPak columns. Recovery from both procedures approached 70-80% of the spiked amount, though the SepPak columns were more efficient. C(18)-SepPak columns were used to sequentially separate oxytocin from AVP by eluting oxytocin first with 98% acetone followed by elution of AVP with 80% acetonitrile. Surface-enhanced laser desorption-ionization time-of-flight mass spectrometry (SELDI-TOF MS) was used to analyze oxytocin and AVP extracted with C(18)-SepPak columns from an autistic patient's plasma sample. We conclude that C(18)-SepPaks provide more consistent and efficient peptide extraction from serum or plasma that augments both quantitative and qualitative analysis by radioimmunoassay and SELDI-TOF MS." @default.
- W2013235514 created "2016-06-24" @default.
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- W2013235514 date "2003-07-01" @default.
- W2013235514 modified "2023-09-25" @default.
- W2013235514 title "Extraction of oxytocin and arginine–vasopressin from serum and plasma for radioimmunoassay and surface-enhanced laser desorption–ionization time-of-flight mass spectrometry" @default.
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- W2013235514 doi "https://doi.org/10.1016/s1570-0232(03)00284-8" @default.
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