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- W2013259585 abstract "Abstract Bacillus megaterium is widely used as companion bacterium in the two-step biosynthesis of 2-keto- l -gulonic acid (2-KLG) by Ketogulonicigenium vulgare . To screen efficiently target companion strains from large numbers of random mutants, a screen method based on spectrophotometry and 24-well microtiter plates was developed and validated on an integrated library of 450 transposon random insertional mutants and two sporulation-defective mutants. The co-culture processes were classified into three groups (low, intermediate and high performance) by K-mean clustering analysis. In addition, mutant m71 was successfully screened out from the library. The substrate conversion ratio of m71 and K. vulgare co-culture process after 72 h was decreased by about 38% compared with that of the wild-type co-culture process in 750 ml flasks. These results indicated that the proposed high throughput method is feasible for screening target companions for the co-culture process of 2-KLG biosynthesis." @default.
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- W2013259585 date "2012-09-01" @default.
- W2013259585 modified "2023-10-16" @default.
- W2013259585 title "A high throughput method to screen companion bacterium for 2-keto-l-gulonic acid biosynthesis by co-culturing Ketogulonicigenium vulgare" @default.
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- W2013259585 doi "https://doi.org/10.1016/j.procbio.2012.05.010" @default.
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