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• W2013266323 abstract "Introduction: The purpose of the present study was to develop a simple method for the simultaneous determination of mepivacaine, tetracaine, and p-butylaminobenzoic acid (BABA) in human plasma using high-performance liquid chromatography (HPLC) with a multiwavelength detector. Methods: Human blood samples containing heparin, as an anticoagulant, and physostigmine (100 μg/ml), as an anticholinesterase, or human plasma containing physostigmine were spiked with various concentrations of mepivacaine, tetracaine and, in some cases, BABA. Blood samples were centrifuged and plasma was deproteinized with trifluoroacetic acid (TFA; 7%). After centrifugation, the pH was adjusted to 4.5 and an aliquot of 20, 50 or 100 μl was injected into the HPLC apparatus. The detection was done simultaneously at wavelengths of 214 and 300 nm. Analytical chromatography was done on a Waters μBondapak C18 reverse-phase column (3.9×300 mm; particle size 10 μm) with a 30-min increasing linear gradient of 20–40% acetonitrile+0.05% TFA in H2O+0.05% TFA at a flow rate of 1 ml/min. The Waters HPLC system included two pumps, an automatic injector, a column oven, and a M490 multiwavelength detector. Quantification was done using integration of peak areas with peaks of authentic mepivacaine, tetracaine, and BABA standards. Results: Calibration curves for standards of mepivacaine, tetracaine, and BABA were linear in the concentration ranges from 0.1 to 100 μg/ml, and the correlation coefficients exceeded .99 for all compounds. The lower limits of detection were 100 ng/ml for mepivacaine and 50 ng/ml for tetracaine and BABA. The yields for mepivacaine, tetracaine, and BABA were 91±2.1%, 82±3.3%, and 88±2.0% (mean±S.E.M., n=6), respectively. Degradation of tetracaine by human plasma at 37 °C was inhibited by physostigmine. Discussion: The method is sensitive enough to allow blood concentration determinations of mepivacaine and tetracaine or its metabolite, BABA, following local anesthesia induced by these two drugs, especially when their toxic effect may be present. This method also may be useful in forensic medicine for determination of the cause of death after local anesthesia with mepivacaine and/or tetracaine." @default.
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• W2013266323 date "2001-11-01" @default.
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• W2013266323 title "Simultaneous determination of mepivacaine, tetracaine, and p-butylaminobenzoic acid by high-performance liquid chromatography" @default.
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• W2013266323 doi "https://doi.org/10.1016/s1056-8719(02)00171-5" @default.
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