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• W2013267360 abstract "Ly‐6E.1 antigen was proposed as a regulatory molecule of T lymphocyte activation, a hematopoietic stem cell marker, a memory cell marker, and an adhesion molecule. Though there were several reports suggesting the presence of Ly‐6 ligand, the characterization of the ligand was not yet performed. As an attempt to screen the expression of Ly‐6E.1 ligand, we prepared a probe for detecting Ly‐6E.1 ligand by producing a fusion protein between Ly‐6E.1 and hlgC γ1. A mammalian cell expression vector with Ly‐6E.1/hlgC γ1 chimeric cDNA was transfected in SP2/0‐Ag14 myeloma cells, and stable transfectants were selected. The fusion protein was produced as a dimer and maintained the epitopes for monoclonal antibodies specific for Ly‐6E.1 and for anti‐human IgG antibody. The purified fusion protein through Gammabind G column was used for FACS analyses for the expression of Ly‐6E.1 ligand. The fusion protein interacted with several cell lines originating from B cells, T cells, or monocytes. The fusion protein also stron..." @default.
• W2013267360 created "2016-06-24" @default.
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• W2013267360 date "1997-01-01" @default.
• W2013267360 modified "2023-09-26" @default.
• W2013267360 title "Expression analysis of the ligand to ly‐6e.1 mouse hematopoietic stem cell antigen" @default.
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• W2013267360 doi "https://doi.org/10.1080/12265071.1997.9647363" @default.
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