Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013270561> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W2013270561 endingPage "179" @default.
- W2013270561 startingPage "179" @default.
- W2013270561 abstract "We studied, for the first time, characterization of the invertase expressed in the methylotrophic yeasts Hansenula polymorpha and Pichia pastoris in terms of enzyme conformational stability and structural behaviour induced by temperature as a function of pH using enzymic assays, differential scanning calorimetry, fluorescence and CD. The enzyme produced in both hosts was very stable over a broad range of pH values, keeping its enzymic activity and structure above 60 °C. Thermal denaturation, as measured by differential scanning calorimetry, was always irreversible. However, the fact that scanning rate had no effect on the calorimetric curves gave us the chance to analyse the data from a thermodynamic point of view. The conformational stabilities were essentially identical under the experimental conditions studied, but stability was always slightly higher in the enzyme expressed in H. polymorpha . This fact indicates that the greater degree of glycosylation of this enzyme form contributed to its increased global stability. Reactivation upon heating at 80 °C depends on protein concentration, suggesting that irreversibility could be associated with slow refolding kinetics at high protein concentration." @default.
- W2013270561 created "2016-06-24" @default.
- W2013270561 creator A5011805768 @default.
- W2013270561 creator A5047602351 @default.
- W2013270561 creator A5052762165 @default.
- W2013270561 creator A5054100219 @default.
- W2013270561 date "2000-12-01" @default.
- W2013270561 modified "2023-09-27" @default.
- W2013270561 title "Characterization of recombinant invertase expressed in methylotrophic yeasts" @default.
- W2013270561 doi "https://doi.org/10.1042/ba20000064" @default.
- W2013270561 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11115390" @default.
- W2013270561 hasPublicationYear "2000" @default.
- W2013270561 type Work @default.
- W2013270561 sameAs 2013270561 @default.
- W2013270561 citedByCount "19" @default.
- W2013270561 countsByYear W20132705612013 @default.
- W2013270561 countsByYear W20132705612014 @default.
- W2013270561 countsByYear W20132705612015 @default.
- W2013270561 countsByYear W20132705612016 @default.
- W2013270561 countsByYear W20132705612017 @default.
- W2013270561 countsByYear W20132705612018 @default.
- W2013270561 countsByYear W20132705612019 @default.
- W2013270561 countsByYear W20132705612020 @default.
- W2013270561 countsByYear W20132705612021 @default.
- W2013270561 crossrefType "journal-article" @default.
- W2013270561 hasAuthorship W2013270561A5011805768 @default.
- W2013270561 hasAuthorship W2013270561A5047602351 @default.
- W2013270561 hasAuthorship W2013270561A5052762165 @default.
- W2013270561 hasAuthorship W2013270561A5054100219 @default.
- W2013270561 hasConcept C104317684 @default.
- W2013270561 hasConcept C121332964 @default.
- W2013270561 hasConcept C13965031 @default.
- W2013270561 hasConcept C148898269 @default.
- W2013270561 hasConcept C175188612 @default.
- W2013270561 hasConcept C178790620 @default.
- W2013270561 hasConcept C181199279 @default.
- W2013270561 hasConcept C185592680 @default.
- W2013270561 hasConcept C204328495 @default.
- W2013270561 hasConcept C2776923230 @default.
- W2013270561 hasConcept C2777313579 @default.
- W2013270561 hasConcept C2778252681 @default.
- W2013270561 hasConcept C2781216036 @default.
- W2013270561 hasConcept C2781372317 @default.
- W2013270561 hasConcept C39519442 @default.
- W2013270561 hasConcept C40767141 @default.
- W2013270561 hasConcept C41183919 @default.
- W2013270561 hasConcept C43617362 @default.
- W2013270561 hasConcept C55493867 @default.
- W2013270561 hasConcept C56856141 @default.
- W2013270561 hasConcept C59061564 @default.
- W2013270561 hasConcept C62520636 @default.
- W2013270561 hasConcept C97355855 @default.
- W2013270561 hasConceptScore W2013270561C104317684 @default.
- W2013270561 hasConceptScore W2013270561C121332964 @default.
- W2013270561 hasConceptScore W2013270561C13965031 @default.
- W2013270561 hasConceptScore W2013270561C148898269 @default.
- W2013270561 hasConceptScore W2013270561C175188612 @default.
- W2013270561 hasConceptScore W2013270561C178790620 @default.
- W2013270561 hasConceptScore W2013270561C181199279 @default.
- W2013270561 hasConceptScore W2013270561C185592680 @default.
- W2013270561 hasConceptScore W2013270561C204328495 @default.
- W2013270561 hasConceptScore W2013270561C2776923230 @default.
- W2013270561 hasConceptScore W2013270561C2777313579 @default.
- W2013270561 hasConceptScore W2013270561C2778252681 @default.
- W2013270561 hasConceptScore W2013270561C2781216036 @default.
- W2013270561 hasConceptScore W2013270561C2781372317 @default.
- W2013270561 hasConceptScore W2013270561C39519442 @default.
- W2013270561 hasConceptScore W2013270561C40767141 @default.
- W2013270561 hasConceptScore W2013270561C41183919 @default.
- W2013270561 hasConceptScore W2013270561C43617362 @default.
- W2013270561 hasConceptScore W2013270561C55493867 @default.
- W2013270561 hasConceptScore W2013270561C56856141 @default.
- W2013270561 hasConceptScore W2013270561C59061564 @default.
- W2013270561 hasConceptScore W2013270561C62520636 @default.
- W2013270561 hasConceptScore W2013270561C97355855 @default.
- W2013270561 hasIssue "3" @default.
- W2013270561 hasLocation W20132705611 @default.
- W2013270561 hasLocation W20132705612 @default.
- W2013270561 hasOpenAccess W2013270561 @default.
- W2013270561 hasPrimaryLocation W20132705611 @default.
- W2013270561 hasRelatedWork W1974455305 @default.
- W2013270561 hasRelatedWork W1996317655 @default.
- W2013270561 hasRelatedWork W2013270561 @default.
- W2013270561 hasRelatedWork W2023763937 @default.
- W2013270561 hasRelatedWork W2043862380 @default.
- W2013270561 hasRelatedWork W2071178103 @default.
- W2013270561 hasRelatedWork W2094550178 @default.
- W2013270561 hasRelatedWork W2183456059 @default.
- W2013270561 hasRelatedWork W2520526539 @default.
- W2013270561 hasRelatedWork W4297141779 @default.
- W2013270561 hasVolume "32" @default.
- W2013270561 isParatext "false" @default.
- W2013270561 isRetracted "false" @default.
- W2013270561 magId "2013270561" @default.
- W2013270561 workType "article" @default.