FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013275370> ?p ?o ?g. }

• W2013275370 endingPage "159" @default.
• W2013275370 startingPage "145" @default.
• W2013275370 abstract "In invertebrates, like Hydra and sea urchins, evidence for a functional cannabinoid system was described. The partial characterization of a putative CB1 cannabinoid receptor in the leech Hirudo medicinalis led us to investigate the presence of a complete endogenous cannabinoid system in this organism. By using gas chromatography-mass spectrometry, we demonstrate the presence of the endocannabinoids anandamide (N-arachidonoylethanolamine, 21.5+/-0.7 pmol/g) and 2-arachidonoyl-glycerol (147.4+/-42.7 pmol/g), and of the biosynthetic precursor of anandamide, N-arachidonylphosphatidyl-ethanolamine (16.5+/-3.3 pmol/g), in the leech central nervous system (CNS). Anandamide-related molecules such as N-palmitoylethanolamine (32.4+/-1.6 pmol/g) and N-linolenoylethanolamine (5.8 pmol/g) were also detected. We also found an anandamide amidase activity in the leech CNS cytosolic fraction with a maximal activity at pH 7 and little sensitivity to typical fatty acid amide hydrolase (FAAH) inhibitors. Using an antiserum directed against the amidase signature sequence, we focused on the identification and the localization of the leech amidase. Firstly, leech nervous system protein extract was subjected to Western blot analysis, which showed three immunoreactive bands at ca. approximately 42, approximately 46 and approximately 66 kDa. The former and latter bands were very faint and were also detected in whole homogenates from the coelenterate Hydra vulgaris, where the presence of CB1-like receptors, endocannabinoids and a FAAH-like activity was reported previously. Secondly, amidase immunocytochemical detection revealed numerous immunoreactive neurons in the CNS of three species of leeches. In addition, we observed that leech amidase-like immunoreactivity matches to a certain extent with CB1-like immunoreactivity. Finally, we also found that stimulation by anandamide of this receptor leads, as in mammals, to inhibition of cAMP formation, although this effect appeared to be occurring through the previously described anandamide-induced and CB1-mediated activation of nitric oxide release. Taken together, these results suggest the existence of a complete and functional cannabinoid system in leeches." @default.
• W2013275370 created "2016-06-24" @default.
• W2013275370 creator A5003372092 @default.
• W2013275370 creator A5003680424 @default.
• W2013275370 creator A5007484138 @default.
• W2013275370 creator A5039090707 @default.
• W2013275370 creator A5052418049 @default.
• W2013275370 creator A5062572691 @default.
• W2013275370 creator A5063035120 @default.
• W2013275370 creator A5076395210 @default.
• W2013275370 creator A5080408150 @default.
• W2013275370 creator A5082040442 @default.
• W2013275370 date "2001-03-01" @default.
• W2013275370 modified "2023-10-11" @default.
• W2013275370 title "Evidence for an endocannabinoid system in the central nervous system of the leech Hirudo medicinalis" @default.
• W2013275370 cites W109761354 @default.
• W2013275370 cites W1685180105 @default.
• W2013275370 cites W1882501940 @default.
• W2013275370 cites W1966451239 @default.
• W2013275370 cites W1969338731 @default.
• W2013275370 cites W1969592824 @default.
• W2013275370 cites W1969762460 @default.
• W2013275370 cites W1973196998 @default.
• W2013275370 cites W1974651773 @default.
• W2013275370 cites W1978379381 @default.
• W2013275370 cites W1983287368 @default.
• W2013275370 cites W1985882985 @default.
• W2013275370 cites W1989073099 @default.
• W2013275370 cites W1997203123 @default.
• W2013275370 cites W2000361888 @default.
• W2013275370 cites W2003594380 @default.
• W2013275370 cites W2004610487 @default.
• W2013275370 cites W2006730284 @default.
• W2013275370 cites W2011344766 @default.
• W2013275370 cites W2013961851 @default.
• W2013275370 cites W2016164238 @default.
• W2013275370 cites W2017972513 @default.
• W2013275370 cites W2020678194 @default.
• W2013275370 cites W2020827621 @default.
• W2013275370 cites W2024653583 @default.
• W2013275370 cites W2029004423 @default.
• W2013275370 cites W2048154756 @default.
• W2013275370 cites W2051741667 @default.
• W2013275370 cites W2053028764 @default.
• W2013275370 cites W2056188558 @default.
• W2013275370 cites W2056416370 @default.
• W2013275370 cites W2065512356 @default.
• W2013275370 cites W2066350084 @default.
• W2013275370 cites W2067864509 @default.
• W2013275370 cites W2075894678 @default.
• W2013275370 cites W2083371696 @default.
• W2013275370 cites W2085741941 @default.
• W2013275370 cites W2087463715 @default.
• W2013275370 cites W2088747149 @default.
• W2013275370 cites W2089478925 @default.
• W2013275370 cites W2094435043 @default.
• W2013275370 cites W2100837269 @default.
• W2013275370 cites W2115808149 @default.
• W2013275370 cites W2116707337 @default.
• W2013275370 cites W2132470832 @default.
• W2013275370 cites W2134297812 @default.
• W2013275370 cites W2137704643 @default.
• W2013275370 cites W2144237856 @default.
• W2013275370 cites W2152872852 @default.
• W2013275370 cites W2157065782 @default.
• W2013275370 cites W2247029019 @default.
• W2013275370 cites W2324311973 @default.
• W2013275370 doi "https://doi.org/10.1016/s0169-328x(00)00290-4" @default.
• W2013275370 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11245916" @default.
• W2013275370 hasPublicationYear "2001" @default.
• W2013275370 type Work @default.
• W2013275370 sameAs 2013275370 @default.
• W2013275370 citedByCount "62" @default.
• W2013275370 countsByYear W20132753702012 @default.
• W2013275370 countsByYear W20132753702013 @default.
• W2013275370 countsByYear W20132753702014 @default.
• W2013275370 countsByYear W20132753702015 @default.
• W2013275370 countsByYear W20132753702016 @default.
• W2013275370 countsByYear W20132753702017 @default.
• W2013275370 countsByYear W20132753702018 @default.
• W2013275370 countsByYear W20132753702019 @default.
• W2013275370 countsByYear W20132753702020 @default.
• W2013275370 countsByYear W20132753702021 @default.
• W2013275370 countsByYear W20132753702022 @default.
• W2013275370 countsByYear W20132753702023 @default.
• W2013275370 crossrefType "journal-article" @default.
• W2013275370 hasAuthorship W2013275370A5003372092 @default.
• W2013275370 hasAuthorship W2013275370A5003680424 @default.
• W2013275370 hasAuthorship W2013275370A5007484138 @default.
• W2013275370 hasAuthorship W2013275370A5039090707 @default.
• W2013275370 hasAuthorship W2013275370A5052418049 @default.
• W2013275370 hasAuthorship W2013275370A5062572691 @default.
• W2013275370 hasAuthorship W2013275370A5063035120 @default.
• W2013275370 hasAuthorship W2013275370A5076395210 @default.
• W2013275370 hasAuthorship W2013275370A5080408150 @default.
• W2013275370 hasAuthorship W2013275370A5082040442 @default.
• W2013275370 hasConcept C136764020 @default.
• W2013275370 hasConcept C148001335 @default.

• next