Matches in SemOpenAlex for { <https://semopenalex.org/work/W2013280912> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W2013280912 endingPage "2165" @default.
- W2013280912 startingPage "2160" @default.
- W2013280912 abstract "Chloroflexus aurantiacus J-10-fl was found to contain two types (protease I and protease II) of thermostable proteases which were separated by Butyl-Toyopearl 650 M chromatography. Protease I was purified to electrophoretic homogeneity from the culture broth of C. aurantiacus J-10-fl. The molecular mass of protease I was estimated to be approximately 66 kDa by SDS-PAGE, and the value of approximately 66 kDa was also obtained by the Hedrick-Smith method, indicating that protease I was a monomer. The isoelectric point was 6.2. Protease I activity was inhibited by metalloprotease inhibitors such as EDTA, EGTA, and o-phenanthroline. The optimum pH for the activity of protease I was around 8.0. Addition of Ca2+ increased the pH and heat stabilities of protease I. The activity was stable between pH 4.0-11.0 and up to 75 degrees C, and the maximum activity was observed at 70 degrees C in the presence of 2 mM CaCl2. Protease I was resistant to the treatment by denaturing reagents (8 M urea or 1% SDS) at pH 8.0 and 20 degrees C for 24 h. The sites of cleavage in oxidized insulin B chain by protease I were similar to those by other microbial neutral metalloproteases. Elastase activity of protease I was not detected." @default.
- W2013280912 created "2016-06-24" @default.
- W2013280912 creator A5002716298 @default.
- W2013280912 creator A5012112585 @default.
- W2013280912 creator A5018047839 @default.
- W2013280912 creator A5082654587 @default.
- W2013280912 date "1993-01-01" @default.
- W2013280912 modified "2023-10-18" @default.
- W2013280912 title "Purification and Characterization of a Thermostable Neutral Metalloprotease I from<i>Chloroflexus aurantiacus</i>J-10-fl" @default.
- W2013280912 cites W1600370803 @default.
- W2013280912 doi "https://doi.org/10.1271/bbb.57.2160" @default.
- W2013280912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7764368" @default.
- W2013280912 hasPublicationYear "1993" @default.
- W2013280912 type Work @default.
- W2013280912 sameAs 2013280912 @default.
- W2013280912 citedByCount "10" @default.
- W2013280912 countsByYear W20132809122013 @default.
- W2013280912 countsByYear W20132809122020 @default.
- W2013280912 countsByYear W20132809122021 @default.
- W2013280912 crossrefType "journal-article" @default.
- W2013280912 hasAuthorship W2013280912A5002716298 @default.
- W2013280912 hasAuthorship W2013280912A5012112585 @default.
- W2013280912 hasAuthorship W2013280912A5018047839 @default.
- W2013280912 hasAuthorship W2013280912A5082654587 @default.
- W2013280912 hasConcept C181199279 @default.
- W2013280912 hasConcept C182220744 @default.
- W2013280912 hasConcept C185592680 @default.
- W2013280912 hasConcept C2776714187 @default.
- W2013280912 hasConcept C43617362 @default.
- W2013280912 hasConcept C55493867 @default.
- W2013280912 hasConcept C55728118 @default.
- W2013280912 hasConcept C58121356 @default.
- W2013280912 hasConcept C86803240 @default.
- W2013280912 hasConcept C96294017 @default.
- W2013280912 hasConceptScore W2013280912C181199279 @default.
- W2013280912 hasConceptScore W2013280912C182220744 @default.
- W2013280912 hasConceptScore W2013280912C185592680 @default.
- W2013280912 hasConceptScore W2013280912C2776714187 @default.
- W2013280912 hasConceptScore W2013280912C43617362 @default.
- W2013280912 hasConceptScore W2013280912C55493867 @default.
- W2013280912 hasConceptScore W2013280912C55728118 @default.
- W2013280912 hasConceptScore W2013280912C58121356 @default.
- W2013280912 hasConceptScore W2013280912C86803240 @default.
- W2013280912 hasConceptScore W2013280912C96294017 @default.
- W2013280912 hasIssue "12" @default.
- W2013280912 hasLocation W20132809121 @default.
- W2013280912 hasLocation W20132809122 @default.
- W2013280912 hasOpenAccess W2013280912 @default.
- W2013280912 hasPrimaryLocation W20132809121 @default.
- W2013280912 hasRelatedWork W1485474307 @default.
- W2013280912 hasRelatedWork W1834258531 @default.
- W2013280912 hasRelatedWork W1976608269 @default.
- W2013280912 hasRelatedWork W1991517410 @default.
- W2013280912 hasRelatedWork W2016330342 @default.
- W2013280912 hasRelatedWork W2026960302 @default.
- W2013280912 hasRelatedWork W2052067084 @default.
- W2013280912 hasRelatedWork W2114609271 @default.
- W2013280912 hasRelatedWork W2164102584 @default.
- W2013280912 hasRelatedWork W2491451478 @default.
- W2013280912 hasVolume "57" @default.
- W2013280912 isParatext "false" @default.
- W2013280912 isRetracted "false" @default.
- W2013280912 magId "2013280912" @default.
- W2013280912 workType "article" @default.